Cell Signaling Technology

Product Pathways - Metabolism

HMOX2/HO-2 Antibody #12226

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Endogenous 36 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

HMOX2/HO-2 Antibody recognizes endogenous levels of total HMOX2/HO-2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Met262 of human HMOX2/HO-2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from NCI-H1299, Jurkat, and PANC-1 cells using HMOX2/HO-2 Antibody.

Background

Heme oxygenases (HMOX or HO) catalyze the rate-limiting step of the oxidative degradation of heme into iron, carbon monoxide, and biliverdin (1). Biliverdin is then converted to bilirubin (2). Heme is a strong pro-oxidant whereas bilirubin is a strong antioxidant (2). Research studies suggest disregulation of heme oxygenases may contribute to oxidative stress-related diseases (2). There are three isozymes of heme oxygenases: HMOX1/HO-1, HMOX2/HO-2, and HMOX3/HO-3 (1,2). HMOX1/HO-1 is inducible by heme and other stress stimuli (1,3). HMOX2/HO-2 and HMOX3/HO-3 are constitutively expressed (1,3).

  1. Piotrkowski, B. et al. (2009) J Endocrinol 203, 155-65.
  2. Synowiec, E. et al. (2012) Mol Biol Rep 39, 2081-7.
  3. Otterbein, L.E. and Choi, A.M. (2000) Am J Physiol Lung Cell Mol Physiol 279, L1029-37.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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