Cell Signaling Technology

Product Pathways - Adhesion

Syndecan 4 Antibody #12236

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 29 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Syndecan 4 Antibody recognizes endogenous levels of total Syndecan 4 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro103 of human Syndecan 4 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat and HaCaT cells using Syndecan 4 Antibody.

Background

Syndecans are a family of type 1 transmembrane heparan sulphate proteoglycans comprising 4 members in mammals (SDC-1 to -4) (1) encoded by four syndecan genes. Syndecans are involved in embryonic development, tumorigenesis, and angiogenesis (2). The extracellular domain harbors attachment sites for heparan sulfate and chondroitin sulfate chains, facilitating interaction with an array of proteins including a plethora of growth factors. In addition, the hydrophobic C-terminal intracellular domain can interact with proteins containing a PDZ domain (2). These interactions place syndecans as important integrators of membrane signaling (3). Syndecans undergo proteolytic cleavage causing the release of their extracellular domain (shedding), converting the membrane-bound proteins into soluble molecular effectors (4).

Syndecan 4 is expressed ubiquitously and is enriched in the focal adhesions of various adherent cell types (5). Syndecan 4 controls focal adhesion formation and regulates cell migration through interaction with Rho family GTPase and protein kinase C family members (6-11). Phosphorylation of the cytoplasmic domain is linked to ectodomain shedding of Syndecan 4, both of which contribute to the regulation of cytokinesis (12).

  1. Couchman, J.R. (2003) Nat Rev Mol Cell Biol 4, 926-37.
  2. Multhaupt, H.A. et al. (2009) J Physiol Pharmacol 60 Suppl 4, 31-8.
  3. Zimmermann, P. and David, G. (1999) FASEB J 13 Suppl, S91-S100.
  4. Manon-Jensen, T. et al. (2010) FEBS J 277, 3876-89.
  5. Woods, A. and Couchman, J.R. (1994) Mol Biol Cell 5, 183-92.
  6. Harrington, E.O. et al. (1997) J Biol Chem 272, 7390-7.
  7. Oh, E.S. et al. (1997) J Biol Chem 272, 8133-6.
  8. Woods, A. and Couchman, J.R. (1992) J Cell Sci 101 ( Pt 2), 277-90.
  9. Longley, R.L. et al. (1999) J Cell Sci 112 ( Pt 20), 3421-31.
  10. Woods, A. et al. (2000) Arch Biochem Biophys 374, 66-72.
  11. Bishop, A.L. and Hall, A. (2000) Biochem J 348 Pt 2, 241-55.
  12. Keller-Pinter, A. et al. (2010) Cell Mol Life Sci 67, 1881-94.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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