Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Ca / cAMP / Lipid Signaling

Calreticulin (D3E6) XP® Rabbit mAb #12238

Applications Reactivity Sensitivity MW (kDa) Isotype
W IF-IC H M R Endogenous 55 Rabbit IgG

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Calreticulin (D3E6) XP® Rabbit mAb recognizes endogenous levels of total calreticulin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human calreticulin protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from SH-SY5Y and KNRK cells using Calreticulin (D3E6) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of NIH/3T3 cells using Calreticulin (D3E6) XP® Rabbit mAb (green) and β-Actin (13E5) Rabbit mAb (Alexa Fluor® 555 Conjugate) #8046 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Calcium is a universal signaling molecule involved in many cellular functions such as cell motility, metabolism, protein modification, protein folding, and apoptosis. Calcium is stored in the endoplasmic reticulum (ER), where it is buffered by calcium binding chaperones such as calnexin and calreticulin, and is released via the IP3 Receptor channel (1). Calreticulin also functions as an ER chaperone that ensures proper folding and quality control of newly synthesized glycoproteins. As such, calreticulin presumably does not alter protein folding but regulates proper timing for efficient folding and subunit assembly. Furthermore, calreticulin retains proteins in non-native conformation within the ER and targets them for degradation (2,3).

  1. Groenendyk, J. et al. (2004) Mol Cells 17, 383-9.
  2. Nauseef, W.M. et al. (1995) J Biol Chem 270, 4741-7.
  3. Williams, D.B. (2006) J Cell Sci 119, 615-23.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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