Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

IL-2 (D7A5) Rabbit mAb #12239

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Endogenous 18 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

IL-2 (D7A5) Rabbit mAb recognizes endogenous levels of total human IL-2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to a fragment of human IL-2.

Western Blotting

Western Blotting

Western blot analysis of recombinant Human Interleukin-2 (hIL-2) (mammalian derived) #5454 using IL-2 (D7A5) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated (-) or treated (+) overnight with TPA #4174 (40 nM), A23187 (2 μM), and Brefeldin A #9972 (BFA, 300 ng/ml, 45 min delay), using IL-2 (D7A5) Rabbit mAb.

Background

Interleukin-2 (IL-2) is a T cell stimulatory cytokine best known for inducing T cell proliferation and NK cell proliferation and activation (1,2). IL-2 also promotes peripheral development of regulatory T cells (Tregs) (3,4). Conversely, IL-2 is involved in the activation-induced cell death (AICD) that is observed post T cell expansion by increasing levels of Fas on CD4+ T cells (5). The effects of IL-2 are mediated through a trimeric receptor complex consisting of IL-2Rα, IL-2Rβ, and the common gamma chain, γc (1,2). IL-2Rα binds exclusively to IL-2 with low affinity and increases the binding affinity of the whole receptor complex including IL-2Rβ and γc subunits. IL-15 also binds to IL-2Rβ (1,2). γc is used by other cytokines including IL-4, IL-7, IL-9, IL-15, and IL-21 (1,2). Binding of IL-2 initiates signaling cascades involving Jak1, Jak3, Stat5, and the PI3K/Akt pathways (1,2).

  1. Ma, A. et al. (2006) Annu Rev Immunol 24, 657-79.
  2. Gaffen, S.L. and Liu, K.D. (2004) Cytokine 28, 109-23.
  3. Fehérvari, Z. et al. (2006) Trends Immunol 27, 109-11.
  4. Antony, P.A. et al. (2006) J Immunol 176, 5255-66.
  5. Jaleco, S. et al. (2003) J Immunol 171, 61-8.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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