Cell Signaling Technology

Product Pathways - NF-kB Signaling

IL-1β (3A6) Mouse mAb #12242

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M Endogenous 17,31 Mouse IgG1

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

IL-1β (3A6) Mouse mAb recognizes endogenous levels of total IL-1β protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human IL-1β protein.

Western Blotting

Western Blotting

Western blot analysis of 1 ng recombinant Human Interleukin-1β (hIL-1β) #8900 and 1 ng recombinant Mouse Interleukin-1β (mIL-1β) #5204 using IL-1β (3A6) Mouse mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from THP-1 cells, untreated (-) or LPS-treated (100 ng/mL, 3 hr; +), using IL-1β (3A6) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from Raw 264.7 cells, untreated (-) or treated with Brefeldin A #9972 (300 ng/mL, last 3 hr of stimulation; +) and LPS (100 ng/mL, 6 hr; +), using IL-1β (3A6) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).


Background

Interleukin-1β (IL-1β), one of the major caspase-1 targets, is a multifunctional cytokine that is involved in a host of immune and proinflammatory responses (1). It is produced primarily by activated monocytes and macrophages. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets (2-6). Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form (7,8). Detection of the 17 kDa mature form of IL-1β is a good indicator of caspase-1 activity.

  1. Dinarello, C.A. (1998) Int. Rev. Immunol. 16, 457-499.
  2. Burns, K. et al. (1998) J. Biol. Chem. 273, 12203-12209.
  3. Cao, Z. et al. (1996) Nature 383, 443-446.
  4. Cao, Z. et al. (1996) Science 271, 1128-1131.
  5. Wesche, H. et al. (1997) Immunity 7, 837-847.
  6. Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-256.
  7. Thornberry, N. A. et al. (1992) Nature 356, 768-774.
  8. Cerretti, D. P. et al. (1992) Science 256, 97-100.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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