Product Pathways - Apoptosis / Autophagy
Bcl-xL Blocking Peptide #1225
Description
This peptide is used to block Bcl-xL (54H6) Rabbit mAb #2764 and Bcl-xL Antibody #2762 reactivity.
Quality Control
The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Bcl-xL (54H6) Rabbit mAb #2764 and Bcl-xL Antibody #2762 signal in both immunohistochemistry and Western blotting.
Western Blotting
Western Blot analysis of extracts from Ramos and Raw cells using Bcl-xL (54H6) Rabbit mAb #2764 (left) or the same antibody preincubated with Bcl-xL Blocking Peptide (right).
Western Blotting
Western Blot analysis of extracts from Ramos and Raw cells using Bcl-xL Antibody #2762 (left) or the same antibody preincubated with Bcl-xL Blocking Peptide (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Bcl-xL Antibody #2762 in the presence of control peptide (left) or Bcl-xL Blocking Peptide (right).
Applications
Use as a blocking reagent to evaluate the specificity of antibody reactivity in Western immunoblotting and immunohistochemistry protocols.
Directions for Use
For Immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 µl total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. For Western blotting, add an equal volume of peptide as volume of antibody used in 10 ml total volume, and incubate at room temperature for 30 minutes before allowing to react with the blot. Recommended antibody dilutions can be found on the Bcl-xL Antibody #2762 and Bcl-xL (54H6) Rabbit mAb #2764 data sheets.
Background
Bcl-xL prevents apoptosis through two different mechanisms: by heterodimerization with an apoptotic protein to inhibit its apoptotic effect (1,2), and by its direct pore-forming effect on the outer membrane of mitochondria which helps maintain a normal membrane state under stress conditions (3). Bcl-xL is phosphorylated by JNK following treatment with microtubule-damaging agents such as paclitaxel, vinblastine and nocodazole (4,5).
- Adams, J.M. and Cory, S. (1998) Science 281, 1322-1326.
- Minn, A.J. et al. (1999) EMBO. J. 18, 632-643.
- Vander Heiden, M.G. et al. (2001) J. Biol. Chem. 276, 19414-19419.
- Fan, M. et al. (2000) J. Biol. Chem. 275, 29980-29985.
- Poruchynsky, M.S. et al. (1998) Cancer Res. 58, 3331-3338.
Application References
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Companion Products
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
