Product Pathways - Apoptosis
Lamin B2 (D8P3U) Rabbit mAb #12255
|12255S||100 µl (10 western blots)||---||In Stock||---|
|12255||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Monkey||Endogenous||68||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
Lamin B2 (D8P3U) Rabbit mAb recognizes endogenous levels of total lamin B2 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys415 of human lamin B2 protein.
Western blot analysis of extracts from various cell lines using Lamin B2 (D8P3U) Rabbit mAb.
Immunoprecipitation of lamin B2 from Jurkat cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Lamin B2 (D8P3U) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Lamin B2 (D8P3U) Rabbit mAb.
Confocal immunofluorescent analysis of HCT 116 cells using Lamin B2 (D8P3U) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Lamins are nuclear membrane structural components that are important in maintaining normal cell functions, such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamins have been subdivided into types A and B. Type-A lamins consist of lamin A and C, which arise from alternative splicing of the lamin A gene LMNA. Lamin A and C are cleaved by caspases into large (41-50 kDa) and small (28 kDa) fragments, which can be used as markers for apoptosis (4,5). Type-B lamins consist of lamin B1 and B2, encoded by separate genes (6-8). Lamin B1 is also cleaved by caspases during apoptosis (9). Research studies have shown that duplication of the lamin B1 gene LMNB1 is correlated with pathogenesis of the neurological disorder adult-onset leukodystrophy (10).
Lamin B2 knockout mice have defects in neuronal development (11) that are distinct from lamin B1 knockout mice (12).
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- Padiath, Q.S. et al. (2006) Nat Genet 38, 1114-23.
- Coffinier, C. et al. (2010) Proc Natl Acad Sci U S A 107, 5076-81.
- Coffinier, C. et al. (2011) Mol Biol Cell 22, 4683-93.
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