Cell Signaling Technology

Product Pathways - Metabolism

G6PD (D5D2) Rabbit mAb #12263

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R Hm Mk Endogenous 58 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

G6PD (D5D2) Rabbit mAb recognizes endogenous levels of total G6PD protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human G6PD protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using G6PD (D5D2) Rabbit mAb.

Background

Glucose-6-phosphate dehydrogenase (G6PD) catalyses the first and rate-limiting step of the pentose phosphate pathway (1). The NADPH generated from this reaction is essential to protect cells from oxidative stress (1). Recent studies have shown that p53 interacts with G6PD and inhibits its activity, therefore suppressing glucose consumption through the pentose phosphate pathway (2). In cancer cells with p53 mutations, the increased glucose consumption is directed towards increased biosynthesis, which is critical for cancer cell proliferation (2).

  1. Au, S.W. et al. (2000) Structure 8, 293-303.
  2. Jiang, P. et al. (2011) Nat Cell Biol 13, 310-6.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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