Cell Signaling Technology

Product Pathways - Neuroscience

Synaptophysin (7H12) Mouse mAb #12270

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R Endogenous 38 Mouse IgG1

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Synaptophysin (7H12) Mouse mAb recognizes endogenous levels of total synaptophysin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human synaptophysin protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various tissues and cell lines using Synaptophysin (7H12) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Background

Synaptophysin (SYP) is a neuronal synaptic vesicle glycoprotein that is expressed in neuroendocrine cells and neoplasms (1). Synaptophysin contains four transmembrane domains that form a hexameric channel or gap junction-like pore (2). Synaptophysin binds to the SNARE protein synaptobrevin/VAMP, which prevents the inclusion of synaptobrevin in the synaptic vesicle fusion complex and creates a pool of synaptobrevin for exocytosis when synapse activity increases (3). Synaptophysin is also responsible for targeting synaptobrevin 2/VAMP2 to synaptic vesicles, a critical component of the fusion complex (4).

  1. Wiedenmann, B. et al. (1986) Proc Natl Acad Sci USA 83, 3500-4.
  2. Valtorta, F. et al. (2004) Bioessays 26, 445-53.
  3. Arthur, C.P. and Stowell, M.H. (2007) Structure 15, 707-14.
  4. Bonanomi, D. et al. (2007) Biochem J 404, 525-34.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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