Cell Signaling Technology

Product Pathways - NF-kB Signaling

CARD9 Antibody (Mouse Preferred) #12283

Applications Reactivity Sensitivity MW (kDa) Source
W IP M Endogenous 64 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CARD9 Antibody (Mouse Preferred) recognizes endogenous levels of total CARD9 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His478 of mouse CARD9 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Raw 264.7 and JAWSII cells using CARD9 Antibody (Mouse Preferred).

IP

IP

Immunoprecipitation of CARD9 from Raw 264.7 cell extracts using Normal Rabbit IgG #2729 (lane 2) or CARD9 Antibody (Mouse Preferred) (lane 3). Lane 1 is 10% input. Western blot analysis was performed using CARD9 Antibody (Mouse Preferred).

Background

CARD9 is a caspase recruitment domain (CARD)-containing adaptor protein expressed by myeloid cells (1,2). It is required for antifungal immunity downstream of pathogen detection by C-type lectin receptors (CLRs) such as Dectin-1 (3,4). Recognition of carbohydrates on fungal cell walls by CLRs leads to activation of the tyrosine kinase Syk, followed by activation of PKCδ (5,6). PKCδ phosphorylates CARD9, enabling the assembly of a complex containing CARD9 and Bcl10 (6). This complex activates NF-κB, resulting in upregulation of inflammatory cytokines important for initiation of adaptive immunity (3,4,6,7). CARD9 was also shown to be important for the induction of IL-1β, downstream of the viral nucleic acid sensor RIG-I, as well as for the generation of reactive oxygen species important for bacterial killing by macrophages (2,8).

  1. Bertin, J. et al. (2000) J Biol Chem 275, 41082-6.
  2. Hsu, Y.M. et al. (2007) Nat Immunol 8, 198-205.
  3. Gross, O. et al. (2006) Nature 442, 651-6.
  4. Bi, L. et al. (2010) J Biol Chem 285, 25969-77.
  5. Rogers, N.C. et al. (2005) Immunity 22, 507-17.
  6. Strasser, D. et al. (2012) Immunity 36, 32-42.
  7. LeibundGut-Landmann, S. et al. (2007) Nat Immunol 8, 630-8.
  8. Wu, W. et al. (2009) Nat Immunol 10, 1208-14.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products