Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Protor-2 (D19A5) Rabbit mAb #12288

Applications Reactivity Sensitivity MW (kDa) Isotype
W H Mk Endogenous 42 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Protor-2 (D19A5) Rabbit mAb recognizes endogenous levels of total protor-2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human protor-2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from DU 145, Hep G2, and HeLa cells using Protor-2 (D19A5) Rabbit mAb.

Background

Protein observed with rictor-1 (protor-1) and protor-2 both interact with rictor and were identified as components of mTORC2 (1). The absence of protor-1 or protor-2 does not affect the assembly of other mTORC2 components into an active complex (2). Protor-1 was shown to be essential for the activation of SGK1 mediated by mTORC2 (2). Protor-2 plays a role in tristetraprolin-mediated mRNA turnover (3).

  1. Pearce, L.R. et al. (2007) Biochem J 405, 513-22.
  2. Pearce, L.R. et al. (2011) Biochem J 436, 169-79.
  3. Holmes, B. et al. (2012) Cell Signal 24, 309-15.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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