Cell Signaling Technology

Product Pathways - Translational Control

PKR (D7F7) Rabbit mAb #12297

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Endogenous 74 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PKR (D7F7) Rabbit mAb recognizes endogenous levels of total PKR protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu222 of human PKR protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from PANC-1, RD, and HeLa cells using PKR (D7F7) Rabbit mAb.

IP

IP

Immunoprecipitation of PKR from PANC-1 cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or PKR (D7F7) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using PKR (D7F7) Rabbit mAb.

Background

Protein kinase R (PKR) is transcriptionally induced by interferon and activated by double-stranded RNA (dsRNA). PKR inhibits translation initiation through phosphorylation of the α subunit of the initiation factor eIF2 (eIF2α) and also controls the activation of several transcription factors, such as NF-κB, p53, and the Stats. In addition, PKR mediates apoptosis induced by many different stimuli, such as LPS, TNF-α, viral infection, and serum starvation (1,2). Activation of PKR by dsRNA results in PKR dimerization and autophosphorylation of Thr446 and Thr451 in the activation loop. Substitution of threonine for alanine at position 451 completely inactivated PKR, while a mutant with a threonine to alanine substitution at position 446 was partially active (3). Research studies have implicated PKR activation in the pathologies of neurodegenerative diseases, including Alzheimer's disease (4,5).

  1. Williams, B.R. (1999) Oncogene 18, 6112-6120.
  2. Gil, J. and Esteban, M. (2000) Apoptosis 5, 107-114.
  3. Romano, P. R. et al. (1998) Mol. Cell. Biol. 18, 2282-2297.
  4. Peel, A.L. and Bredesen, D.E. (2003) Neurobiol. Dis. 14, 52-62.
  5. Peel, A.L. (2004) J. Neuropath. Exp. Neurol. 63, 97-105.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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