Cell Signaling Technology

Product Pathways - Lineage Markers

CDX2 (D11D10) Rabbit mAb #12306

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IHC-P IF-IC H Endogenous 38 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CDX2 (D11D10) Rabbit mAb recognizes endogenous levels of total CDX2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human CDX2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CDX2 (D11D10) Rabbit mAb. HeLa extracts show negative or extremely low expression as expected.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CDX2 (D11D10) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon using CDX2 (D11D10) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).


IF-IC

IF-IC

Confocal immunofluorescent analysis of DLD-1 (positive, left) and HeLa (negative, right) cells using CDX2 (D11D10) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

Background

CDX2, a homeobox domain-containing transcription factor, is a master regulator of the trophoectoderm, the layer that gives rise to extra-embryonic tissues in mammalian development (1). CDX2 is also involved in intestinal development (2), and gain of expression or loss of expression has been associated with various human malignancies such as Barret Esophagus (3) and colorectal cancer (4,5). Mouse embryonic stem cells deficient in CDX2 display limited hematopoietic progenitor development and altered Hox gene expression (6), pointing to a role for CDX2 in Hox gene regulation. CDX2 is also implicated in the aberrant expression of Hox genes in human AML cell lines (7).

  1. Ralston, A. and Rossant, J. (2008) Dev Biol 313, 614-29.
  2. Traber, P.G. and Silberg, D.G. (1996) Annu Rev Physiol 58, 275-97.
  3. Shi, X.Y. et al. (2008) Am J Clin Pathol 129, 571-7.
  4. Bakaris, S. et al. (2008) Histol Histopathol 23, 1043-7.
  5. Guo, R.J. et al. (2004) Cancer Biol Ther 3, 593-601.
  6. Wang, Y. et al. (2008) Proc Natl Acad Sci U S A 105, 7756-61.
  7. Fröhling, S. et al. (2007) Cell Cycle 6, 2241-5.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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