Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

KIFC1 Antibody #12313

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Endogenous 75 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

KIFC1 Antibody recognizes endogenous levels of total KIFC1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human KIFC1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using KIFC1 Antibody.

IP

IP

Immunoprecipitation of KIFC1 from HeLa cell extracts using KIFC1 Antibody (lane 2) or Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 3). Lane 1 is 10% input. Western blot was performed using KIFC1 Antibody.

Background

Kinesin superfamily proteins (KIFs) are molecular motors that drive directional, microtubule-dependent intracellular transport of membrane-bound organelles and other macromolecules (e.g. proteins, nucleic acids). The intracellular transport functions of KIFs are fundamentally important for a variety of cellular functions, including mitotic and meiotic division, motility/migration, hormone and neurotransmitter release, and differentiation (1-4). Disruptions to KIF-mediated intracellular transport have been linked with a variety of pathologies, ranging from tumorigenesis to defects in higher order brain function, such as learning and memory (4-6).

KIFC1/HSET is a minus-end directed KIF involved in the processing and movement of early endocytic vesicles (7,8), as well as microtubule crosslinking and spindle assembly (9,10).

  1. Hirokawa, N. et al. (2009) Nat Rev Mol Cell Biol 10, 682-96.
  2. Yu, Y. and Feng, Y.M. (2010) Cancer 116, 5150-60.
  3. Park, J.J. et al. (2008) Mol Endocrinol 22, 989-1005.
  4. Hirokawa, N. et al. (2010) Neuron 68, 610-38.
  5. Yoshimura, Y. et al. (2010) Mol Cell Biol 30, 2206-19.
  6. Hirokawa, N. and Noda, Y. (2008) Physiol Rev 88, 1089-118.
  7. Nath, S. et al. (2007) Mol Biol Cell 18, 1839-49.
  8. Zhu, C. et al. (2005) Mol Biol Cell 16, 3187-99.
  9. Mountain, V. et al. (1999) J Cell Biol 147, 351-66.
  10. Cai, S. et al. (2009) Mol Biol Cell 20, 1348-59.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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