Cell Signaling Technology

Product Pathways - Neuroscience

VGLUT1 Antibody #12331

Applications Reactivity Sensitivity MW (kDa) Source
W M R (H) Endogenous 62 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

VGLUT1 Antibody recognizes endogenous levels of total VGLUT1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human VGLUT1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from rat synaptosome, mouse brain, and rat brain tissue using VGLUT1 Antibody.

Background

Glutamatergic neurons release glutamate, the most common excitatory neurotransmitter. Their synaptic vesicles are filled with glutamate by vesicular glutamate transporters, VGLUTs (1). VGLUT1, also called SLC17A7 for solute carrier family 17 member 7, was first identified as an inorganic phosphate transporter (2). Despite the absence of homology with neurotransmitter transporters, VGLUT1 was later demonstrated to be a glutamate transporter (1) specific to glutamatergic neurons (3). Closely related to VGLUT1, VGLUT2 and VGLUT3 are also involved in glutamate uptake into synaptic vesicles, but define different neuronal subpopulations (4,5). VGLUT1 and VGLUT2 are the most abundant isoforms. VGLUT1 is expressed in the cortex, hippocampus, and cerebellar cortex, while VGLUT2 is mostly found in the thalamus (6,7), and VGLUT3 is expressed in hair cells of the auditory pathway (8).

Research studies have linked VGLUT1 to schizophrenia (9) and depressive disorders (10).

  1. Bellocchio, E.E. et al. (2000) Science 289, 957-60.
  2. Ni, B. et al. (1996) J Neurochem 66, 2227-38.
  3. Takamori, S. et al. (2000) Nature 407, 189-94.
  4. Fremeau, R.T. et al. (2001) Neuron 31, 247-60.
  5. Fremeau, R.T. et al. (2002) Proc Natl Acad Sci U S A 99, 14488-93.
  6. Herzog, E. et al. (2001) J Neurosci 21, RC181.
  7. Kaneko, T. and Fujiyama, F. (2002) Neurosci Res 42, 243-50.
  8. Gillespie, D.C. et al. (2005) Nat Neurosci 8, 332-8.
  9. Oni-Orisan, A. et al. (2008) Biol Psychiatry 63, 766-75.
  10. Uezato, A. et al. (2009) Bipolar Disord 11, 711-25.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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