Cell Signaling Technology

Product Pathways - Neuroscience

TPH-1 (D10C10) Rabbit mAb #12339

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP M (H) Endogenous 51 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

TPH-1 (D10C10) Rabbit mAb recognizes endogenous levels of total TPH-1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TPH-1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length human TPH-1 (hTPH-1; +) and P815 cells, using TPH-1 (D10C10) Rabbit mAb (upper) or α/β-Tubulin Antibody #2148 (lower).

Background

Tryptophan hydroxylase (TPH) is the rate-limiting enzyme in the biosynthesis of serotonin (1) by converting tryptophan to 5-hydroxy-L-tryptophan (2). Two isoforms of TPH exist: TPH-1 is mainly expressed in the periphery, whereas the expression of TPH-2 is restricted to neuronal cells and the central nervous system (3). Most of the serotonin found throughout the body is synthesized by TPH-1 in enterochromaffin cells of the gastrointestinal tract. Targeted disruption of the tph1 gene results in low levels of circulating and tissue serotonin (4).

  1. Hale, M.W. et al. (2011) J Chem Neuroanat 41, 219-26.
  2. Silva, S.R. et al. (2011) Anticancer Res 31, 1153-60.
  3. Walther, D.J. et al. (2003) Science 299, 76.
  4. Amireault, P. et al. (2011) Proc Natl Acad Sci U S A 108, 13141-6.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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