Cell Signaling Technology

Product Pathways - Lineage Markers

MyoD1 Antibody #12344

Applications Reactivity Sensitivity MW (kDa) Source
W IP H (Mk) Endogenous 45 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

MyoD1 Antibody recognizes endogenous levels of total MyoD1 protein. This antibody also detects a 142 kDa protein of unknown origin.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly228 of human MyoD1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from RH30 and RD cells using MyoD1 Antibody.

Background

Myoblast determination protein 1 (MyoD1), also called myogenic factor 3 (Myf3), is a member of the MyoD family of muscle­specific bHLH transcription factors (1). This family is responsible for controlling specification of the muscle cell lineage and is expressed only in skeletal muscle and its precursors. MyoD1 is considered a master regulator of skeletal myogenesis as its expression can induce myogenic differentiation in myoblasts, fibroblasts, and a variety of other cell types (2,3). Through ChIP-sequencing experiments, researchers have discovered that MyoD is associated with the promoters of many genes in muscle cells, but it only regulates a subset of those genes. Recent studies point to regulation of MyoD transcriptional activity via epigenetic mechanisms involving SWI/SNF complexes and Polycomb and Trithorax Group proteins (4-6). Additional influences on muscle development include signal transduction through MAPK, PI3K/Akt, myostatin, NF-κB, and mTOR signaling pathways (5-7).

  1. Berkes, C.A. and Tapscott, S.J. (2005) Semin Cell Dev Biol 16, 585-95.
  2. Tapscott, S.J. (2005) Development 132, 2685-95.
  3. Davis, R.L. et al. (1987) Cell 51, 987-1000.
  4. de la Serna, I.L. et al. (2001) Nat Genet 27, 187-90.
  5. Aziz, A. et al. (2010) Epigenetics 5, 691-5.
  6. Guttridge, D.C. (2004) Curr Opin Clin Nutr Metab Care 7, 443-50.
  7. Ge, Y. and Chen, J. (2012) J Biol Chem, Epub ahead of print.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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