Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Cell Cycle / Checkpoint

ERCC1 (D6G6) XP® Rabbit mAb #12345

Applications Reactivity Sensitivity MW (kDa) Isotype
W IHC-P H M R Mk Endogenous 39 Rabbit IgG

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

ERCC1 (D6G6) XP® Rabbit mAb recognizes endogenous levels of total ERCC1 protein. This antibody also detects ERCC1 splice variants around 30 kDa.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ERCC1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using ERCC1 (D6G6) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ERCC1 (D6G6) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using ERCC1 (D6G6) XP® Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded cell pellets, HeLa (left) or XP2Y0 (right), using ERCC1 (D6G6) XP® Rabbit mAb.

Background

DNA repair systems operate in all living cells to manage a variety of DNA lesions. Nucleotide excision repair (NER) is implemented in cases where bulky helix-distorting lesions occur, such as those brought about by UV and certain chemicals (1). Excision Repair Cross Complementing 1 (ERCC1) forms a complex with XPF, which acts as the 5’ endonuclease required to excise the lesion (2). ERCC1-XPF is also required for repair of DNA interstrand crosslinks (ICLs) (3) and involved in repair of double strand breaks (4). Research studies have shown that expression of ERCC1 is related to survival rate and response to chemotherapeutic drugs in several human cancers including non-small cell lung cancer (NSCLC) (5,6).

  1. Shuck, S.C. et al. (2008) Cell Res 18, 64-72.
  2. McDaniel, L.D. and Schultz, R.A. (2008) Adv Exp Med Biol 637, 65-82.
  3. Niedernhofer, L.J. et al. (2004) Mol Cell Biol 24, 5776-87.
  4. Ahmad, A. et al. (2008) Mol Cell Biol 28, 5082-92.
  5. Zheng, Z. et al. (2007) N Engl J Med 356, 800-8.
  6. Gossage, L. and Madhusudan, S. (2007) Cancer Treat Rev 33, 565-77.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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