Product Pathways - Jak/Stat Pathway
Stat Family Alexa Fluor® 488 Conjugated Antibody Sampler Kit #12348
|12348S||1 Kit (4 x 40 µl)||---||In Stock||---|
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|Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb (Alexa Fluor® 488 Conjugate) #9174||40 µl||F||H, M||Rabbit IgG|
|Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #4323||40 µl||F||H, M, R, Mk||Hm, B, Pg, Hr||Rabbit IgG|
|Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb (Alexa Fluor® 488 Conjugate) #3939||40 µl||F||H, M||R, Mk, B||Rabbit IgG|
|Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate) #2975||40 µl||IF-IC, F||Rabbit IgG|
†Species predicted to react based on 100% sequence homology.
Applications Key: F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey
Flow cytometric analysis of TF-1 cells, untreated (blue) or treated with hGM-CSF #8922 (green), using Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb (Alexa Fluor® 488 Conjugate) #3939.
Flow cytometric analysis of Jurkat cells, untreated (blue) or IFN-α-treated (green), using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #4323 compared to a nonspecific control antibody (red).
Flow cytometric analysis of Jurkat cells, untreated (blue) or IFN-α-treated (green), using Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb (Alexa Fluor® 488 Conjugate) #9174.
The Stat Family Alexa Fluor® 488 Conjugated Antibody Sampler Kit provides an economical means to study the activation status of members of the Stat family of proteins without the need for a fluorescent secondary antibody.
Specificity / Sensitivity
Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of Stat1 only when phosphorylated at Tyr701. This antibody detects phosphorylated Tyr701 of p91 Stat1 and also the p84 splice variant. It does not cross-react with the corresponding phospho-tyrosines of other Stat proteins. Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of Stat3 only when phosphorylated at Tyr705. This antibody does not cross-react with phospho-EGFR or the corresponding phospho-tyrosines of other Stat proteins. Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of Stat5a only when phosphorylated at Tyr694 and Stat5b when phosphorylated at Tyr699.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr701 of human Stat1 protein, Tyr705 of mouse Stat3 protein, or Tyr694 of human Stat5a protein.
Jaks (Janus Kinases) and Stats (Signal Transducers and Activators of Transcription) are utilized by receptors for a wide variety of ligands including cytokines, hormones, growth factors, and neurotransmitters. Jaks, activated via autophosphorylation following ligand-induced receptor aggregation, phosphorylate tyrosine residues on associated receptors, Stat molecules, and other downstream signaling proteins (1,2). The phosphorylation of Stat proteins at conserved tyrosine residues activates SH2-mediated dimerization followed rapidly by nuclear translocation. Stat dimers bind to interferon response element (IRE) and gamma interferon-activated sequence (GAS) DNA elements, resulting in the transcriptional regulation of downstream genes (1,2). The remarkable range and specificity of responses regulated by the Stat family members is determined in part by the tissue-specific expression of different cytokine receptors, Jak and Stat family members (2,3), and by the combinatorial coupling of various Stat members to different receptors. Stat1 is activated in response to a large number of ligands (4) and is essential for responsiveness to IFN-α and IFN-γ (5,6). Stat3 is an important signaling molecule for many cytokines and growth factor receptors (4) and is required for murine fetal development (7). Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone, and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (8,9).
- Darnell, J.E. et al. (1994) Science 264, 1415-21.
- Leonard, W.J. and O'Shea, J.J. (1998) Annu Rev Immunol 16, 293-322.
- Caldenhoven, E. et al. (1996) J Biol Chem 271, 13221-7.
- Heim, M.H. (1999) J Recept Signal Transduct Res 19, 75-120.
- Durbin, J.E. et al. (1996) Cell 84, 443-50.
- Meraz, M.A. et al. (1996) Cell 84, 431-42.
- Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4.
- Gouilleux, F. et al. (1994) EMBO J 13, 4361-9.
- Wakao, H. et al. (1994) EMBO J 13, 2182-91.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
Select rabbit monoclonal antibodies are developed, validated, and produced at CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and in some instances 7,429,487) from Epitomics, Inc.