Cell Signaling Technology

Product Pathways - Adhesion

VCAM-1 Antibody #12367

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Endogenous 95-120 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

VCAM-1 Antibody recognizes endogenous levels of total VCAM-1 protein. This antibody cross-reacts with proteins of unknown origin 48 and 49 kDa.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro354 of human VCAM-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HUVE cells, untreated (-) or treated with Human Tumor Necrosis Factor-α (hTNF-α) #8902 (10 ng/ml, 16 hr; +), using VCAM-1 Antibody (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human VCAM-1 (hVCAM-1-Myc/DDK; +), using VCAM-1 Antibody.

IP

IP

Immunoprecipitation of VCAM-1 from HUVEC extracts, treated with Human Tumor Necrosis Factor-α (hTNF-α) #8902 (10 ng/ml, 16 hr), using Normal Rabbit IgG #2729 (lane 2) or VCAM-1 Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using VCAM-1 Antibody.


Background

VCAM-1 (vascular cell adhesion molecule-1) is a transmembrane glycoprotein containing multiple amino-terminal extracellular Ig-like domains, a transmembrane domain, and a short carboxy-terminal cytoplasmic domain (1). Alternative splicing generates two isoforms of VCAM-1 (2). The role of VCAM-1 during infection and inflammatory diseases is well characterized. Expression of VCAM-1 is induced in endothelial cells by inflammatory cytokines including TNF-α and IL-1β (1). VCAM-1 on endothelial cells interacts with the integrin VLA-4 (α4β1) on leukocytes to mediate migration of circulating leukocytes from the blood across the endothelium and into tissues (3).

  1. Osborn, L. et al. (1989) Cell 59, 1203-11.
  2. Hession, C. et al. (1991) J Biol Chem 266, 6682-5.
  3. Elices, M.J. et al. (1990) Cell 60, 577-84.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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