Product Pathways - Transcription Factors
Thyroid Transcription Factor 1 (D2E8) Rabbit mAb #12373
|12373S||100 µl (10 western blots)||---||In Stock||---|
|12373||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||39, 42||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry)
Species predicted to react based on 100% sequence homology: Monkey.
Specificity / Sensitivity
Thyroid Transcription Factor 1 (D2E8) Rabbit mAb recognizes endogenous levels of total thyroid transcription factor 1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human thyroid transcription factor 1 protein.
Western blot analysis of extracts from H441 (TTF1 positive), TT (TTF1 positive), A549 (TTF1 negative), and HeLa (TTF1 negative) cells using Thyroid Transcription Factor 1 (D2E8) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Confocal immunofluorescent analysis of H441 (left) and A549 (right) cells using Thyroid Transcription Factor 1 (D2E8) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Confocal immunofluorescent analysis of the hypothalamic region of adult mouse brain using Thyroid Transcription Factor 1 (D2E8) Rabbit mAb (green) and Neurofilament-H (RMdO 20) Mouse mAb #2836 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Thyroid Transcription Factor 1 (TTF1, also known as NKX2-1), a member of the NKX homeobox transcription factor family, was initially discovered in the FRTL-5 rat thyroid cell line (1). Subsequent studies have shown that TTF1 plays an important role in differentiation and morphogensis of the developing thyroid, lung, and ventral forebrain (2). TTF1 controls the expression of several genes, some of which are tissue specific, such as: thyroglobulin, thyroperoxidase, and the thyrotropin receptor in the thyroid; and surfactant proteins and clara cell secretory protein in the lung (2,3). Investigators have found that TTF1 is expressed in malignant tumors of the thyroid and lung, and it is commonly used as a marker for both primary and malignant lung cancers (4-6).
- Civitareale, D. et al. (1989) EMBO J 8, 2537-42.
- Boggaram, V. (2009) Clin Sci (Lond) 116, 27-35.
- Yamaguchi, T. et al. (2012) Cancer Cell 21, 348-61.
- Whithaus, K. et al. (2012) Arch Pathol Lab Med 136, 155-62.
- Yoshida, A. et al. (2011) Lung Cancer 72, 309-15.
- Moldvay, J. et al. (2004) Pathol Oncol Res 10, 85-8.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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