Product Pathways - Transcription Factors
Thyroid Transcription Factor 1 (TTF-1) (D2E8) Rabbit mAb #12373
|12373S||100 µl (10 western blots)||---||In Stock||---|
|12373||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||39, 42||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry)
Species predicted to react based on 100% sequence homology: Monkey.
Specificity / Sensitivity
Thyroid Transcription Factor 1 (TTF-1) (D2E8) Rabbit mAb recognizes endogenous levels of total thyroid transcription factor 1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human thyroid transcription factor 1 protein.
Western blot analysis of extracts from TTF-1 positive H441 and TT cells, and TTF-1 negative A549 and HeLa cells, using Thyroid Transcription Factor 1 (TTF-1) (D2E8) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Confocal immunofluorescent analysis of H441 (left) and A549 (right) cells using Thyroid Transcription Factor 1 (TTF-1) (D2E8) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Confocal immunofluorescent analysis of the hypothalamic region of adult mouse brain using Thyroid Transcription Factor 1 (TTF-1) (D2E8) Rabbit mAb (green) and Neurofilament-H (RMdO 20) Mouse mAb #2836 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Thyroid Transcription Factor 1 (TTF-1, also known as NKX2-1), a member of the NKX homeobox transcription factor family, was initially discovered in the FRTL-5 rat thyroid cell line (1). Subsequent studies have shown that TTF-1 plays an important role in differentiation and morphogenesis of the developing thyroid, lung, and ventral forebrain (2). TTF-1 controls the expression of several genes, some of which are tissue specific, such as: thyroglobulin, thyroperoxidase, and the thyrotropin receptor in the thyroid; and surfactant proteins and clara cell secretory protein in the lung (2,3). Investigators have found that TTF-1 is expressed in malignant tumors of the thyroid and lung, and it is commonly used as a marker for both primary and malignant lung cancers (4-6).
- Civitareale, D. et al. (1989) EMBO J 8, 2537-42.
- Boggaram, V. (2009) Clin Sci (Lond) 116, 27-35.
- Yamaguchi, T. et al. (2012) Cancer Cell 21, 348-61.
- Whithaus, K. et al. (2012) Arch Pathol Lab Med 136, 155-62.
- Yoshida, A. et al. (2011) Lung Cancer 72, 309-15.
- Moldvay, J. et al. (2004) Pathol Oncol Res 10, 85-8.
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