Product Pathways - Metabolism
NRF1 Antibody #12381
|12381S||100 µl (10 western blots)||---||In Stock||---|
|12381||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Rat, Monkey||Endogenous||68||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
NRF1 Antibody recognizes endogenous levels of total NRF1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu167 of human NRF1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human NRF1 (hNRF1-Myc; +), using NRF1 Antibody (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunoprecipitation of NRF1 from SH-SY5Y cell extracts using Normal Rabbit IgG #2729 (lane 2) or NRF1 Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using NRF1 Antibody.
Nuclear respiratory factor 1 (NRF1) was identified as a transcription activator for the gene encoding cytochrome c (1). It was later found to play a role in the nuclear control of mitochondrial function (1). PGC-1 induces the expression of NRF1 and NRF2 (2). NRF1, along with the coactivator PGC-1, stimulates the promoter of mitochondrial transcription factor A, which regulates mitochondrial biogenensis and function (2). Recently, C. elegans SKN-1, the ortholog of mammalian NRF1/2/3, was implicated in stress resistance and longevity (3). In C. elegans, SKN-1/NRF is required to mediate the stress resistance and extended life span when either TORC1 or TORC2 is inhibited (4).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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