Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

PI3 Kinase Class II α (D3Q5B) Rabbit mAb #12402

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk Endogenous 180 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PI3 Kinase Class II α (D3Q5B) Rabbit mAb recognizes endogenous levels of total PI3K class II α protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly717 of human PI3K class II α protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from SW620 and HeLa cells using PI3 Kinase Class II α (D3Q5B) Rabbit mAb.

IP

IP

Immunoprecipitation of PI3 Kinase Class II α from HeLa cell extracts, using Normal Rabbit IgG #2729 (lane 2) or PI3 Kinase Class II α Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using PI3 Kinase Class II α (D3Q5B) Rabbit mAb.

Background

Class II phosphatidylinositol 3-kinases (PI3K) contain a C-terminal C2 domain that is unique to the class II isoforms of the PI3K family. This C2 domain mediates protein and phospholipid binding acitivities (1,2). PI3K Class II α generates phosphatidylinositol 3-phosphate (PIP3) and phosphatidylinositol 3,4-bisphosphate (PI(3, 4)P2) from phosphatidylinositol and phosphatidylinositol 4-phosphate (3). PI3K Class II α is located in various intracellular locations such as the trans-Golgi network, endocytic compartments, clathrin-coated vesicles, and nuclear speckles (1,4,5). Research studies have indicated that PI3K Class II α regulates the assembly and distribution of clathrin, resulting in the modulation of clathrin-dependent trafficking and sorting within the trans Golgi network (5,6). PI3K Class II α also mediates translocation of the glucose transporter GLUT4 to the plasma membrane in response to insulin (7). PI3K Class II α has also been shown to regulate neurosecretory granule exocytosis (8) and vascular smooth muscle contraction (9). Unlike other PI3K family members, PI3K Class II α is less sensitive to the PI3K inhibitors wortmannin and LY294002 (3).

  1. Didichenko, S.A. and Thelen, M. (2001) J Biol Chem 276, 48135-42.
  2. Stahelin, R.V. et al. (2006) J Biol Chem 281, 39396-406.
  3. Domin, J. et al. (1997) Biochem J 326 ( Pt 1), 139-47.
  4. Domin, J. et al. (2000) J Biol Chem 275, 11943-50.
  5. Gaidarov, I. et al. (2001) Mol Cell 7, 443-9.
  6. Gaidarov, I. et al. (2005) J Biol Chem 280, 40766-72.
  7. Falasca, M. et al. (2007) J Biol Chem 282, 28226-36.
  8. Wen, P.J. et al. (2008) Mol Biol Cell 19, 5593-603.
  9. Yoshioka, K. et al. (2007) Mol Pharmacol 71, 912-20.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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