Cell Signaling Technology

Product Pathways - MAPK Signaling

SOS1 (D3T7T) Rabbit mAb #12409

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R Hm Mk Endogenous 152 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

SOS1 (D3T7T) Rabbit mAb recognizes endogenous levels of total SOS1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1250 of human SOS1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SOS1 (D3T7T) Rabbit mAb.

Background

Son of sevenless (SOS) was first identified in Drosophila as a guanine nucleotide exchange factor (GEF) for Ras acting downstream of the Sevenless receptor (1). Two closely related homologs of Drosophila SOS are found in mammalian cells: SOS1 and SOS2 (2). SOS1 consists of histone folds, Dbl (DH) and pleckstrin (PH) homology domains, a Ras exchange motif (REM), and Cdc25 homology and polyproline domains (3). SOS1 binds to GRB2, NCK, and other adaptor proteins, and plays an important role in ERK activation downstream of protein tyrosine kinase receptor (RTK). Research studies have identified mutations in the corresponding SOS1 gene of patients with Noonan syndrome, a developmental disorder characterized by short stature, facial dysmorphia, and congenital heart defects (4,5).

  1. Rogge, R.D. et al. (1991) Cell 64, 39-48.
  2. Nimnual, A. and Bar-Sagi, D. (2002) Sci STKE 2002, pe36.
  3. Chardin, P. et al. (1993) Science 260, 1338-43.
  4. Tartaglia, M. et al. (2007) Nat Genet 39, 75-9.
  5. Roberts, A.E. et al. (2007) Nat Genet 39, 70-4.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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