Product Pathways - Metabolism
HNF1α Antibody #12425
|12425S||100 µl||---||In Stock||---|
|12425||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
HNF1α Antibody recognizes endogenous levels of total HNF1α protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human HNF1α protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing DDK-tagged full-length human HNF1α (hHNF1α-DDK; +), using HNF1α Antibody (upper), DYKDDDDK Tag (9A3) Mouse mAb #8146 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunoprecipitation of HNF1α from Hep G2 cell extracts using Normal Rabbit IgG #2729 (lane 2) or HNF1α Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using HNF1α Antibody.
Hepatocyte nuclear factor 1α (HNF1α, also known as TCF1 or MODY3) is a transcription factor that plays a role in the tissue-specific regulation of liver gene expression (1). Research has shown that heterogeneous mutations of HNF1α are linked to maturity onset diabetes of the young (MODY) (2). Recent studies indicate that increased concentrations of free fatty acids can reduce the expression of FoxA2/HNF3β and HNF1α in pancreatic β-cells and lead to their nuclear exclusion, resulting in symptoms of several metabolic syndromes (3).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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