Product Pathways - TGF-beta/Smad Signaling
Phospho-Smad1/5 (Ser463/465) (D5B10) Rabbit mAb (PE Conjugate) #12428
|12428S||100 µl (50 tests)||---||In Stock||---|
|12428||carrier free and custom formulation / quantity||email request|
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|F||1:50||Human, Mouse, Rat, Monkey||Endogenous||60||Rabbit IgG|
Species cross-reactivity is determined by western blot using the unconjugated antibody.
Applications Key: F=Flow Cytometry
Specificity / Sensitivity
Phospho-Smad1/5 (Ser463/465) (D5B10) Rabbit mAb (PE Conjugate) recognizes endogenous levels of Smad1 protein when phosphorylated at Ser465 only, at both Ser463 and Ser465, or at Ser462, Ser463 and Ser465. This antibody also recognizes the endogenous levels of Smad5 protein when phosphorylated at Ser463 only, Ser465 only, at both Ser463 and Ser465, at both Ser462 and Ser465, or at Ser462, Ser463 and Ser465.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser463/465 of human Smad1 and Smad5 protein.
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Smad1/5 (Ser463/465) (D5B10) Rabbit mAb #11971.
Bone morphogenetic proteins (BMPs) constitute a large family of signaling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation, and apoptosis (1,2). BMP receptors are members of the TGF-β family of Ser/Thr kinase receptors. Ligand binding induces multimerization, autophosphorylation, and activation of these receptors (3-5). They subsequently phosphorylate Smad1 at Ser463 and Ser465 in the carboxy-terminal motif SSXS, as well as Smad5 and Smad8 at their corresponding sites. These phosphorylated Smads dimerize with the coactivating Smad4 and translocate to the nucleus, where they stimulate transcription of target genes (5).
MAP kinases and CDKs 8 and 9 phosphorylate residues in the linker region of Smad1, including Ser206. The phosphorylation of Ser206 recruits Smurf1 to the linker region and leads to the degradation of Smad1 (6). Phosphorylation of this site also promotes Smad1 transcriptional action by recruiting YAP to the linker region (7).
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- Kretzschmar, M. et al. (1997) Genes Dev. 11, 984-995.
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- Sapkota, G. et al. (2007) Mol Cell 25, 441-54.
- Alarcón, C. et al. (2009) Cell 139, 757-69.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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