Cell Signaling Technology

Product Pathways - Autophagy Signaling

FIP200 (D10D11) Rabbit mAb #12436

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M (Mk) Endogenous 200 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

FIP200 (D10D11) Rabbit mAb recognizes endogenous levels of total FIP200 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human FIP200 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using FIP200 (D10D11) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from A172 cells, untreated (-) or chloroquine-treated (50 μM, overnight; +) using FIP200 (D10D11) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expresssing full-length human FIP200 (hFIP200; +), using FIP200 (D10D11) Rabbit mAb.


IP

IP

Immunoprecipitation of FIP200 from JJN-3 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or FIP200 (D10D11) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using FIP200 (D10D11) Rabbit mAb.

Background

FIP200 (FAK family kinase-interacting protein of 200 kDa) was identified in a two-hybrid screen with the tyrosine kinase Pyk2 and can inhibit Pyk2 kinase activity as well as related family members (1). FIP200 was later independently identified in a multi-drug resistance screen and named RB1CC1 (RB1-inducible coiled-coil 1) due to its induction by cytotoxic stress and RB1 expression regulation (2). FIP200 function has been linked to apoptosis, cell cycle progression, cell growth, and migration (reviewed in 3). FIP200 has also recently been shown to interact with ULK1 and is required for autophagosome formation (4). FIP200 is part of an ULK1 complex along with Atg13 that is regulated by mTOR and is required for starvation induced autophagy (5-7).

  1. Ueda, H. et al. (2000) J Cell Biol 149, 423-30.
  2. Chano, T. et al. (2002) Oncogene 21, 1295-8.
  3. Gan, B. and Guan, J.L. (2008) Cell Signal 20, 787-94.
  4. Hara, T. et al. (2008) J Cell Biol 181, 497-510.
  5. Hosokawa, N. et al. (2009) Mol Biol Cell 20, 1981-91.
  6. Jung, C.H. et al. (2009) Mol Biol Cell 20, 1992-2003.
  7. Ganley, I.G. et al. (2009) J Biol Chem 284, 12297-305.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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