Product Pathways - Protein Stability
S5a/PSMD4 (D20B2) Rabbit mAb #12441
PhosphoSitePlus® protein, site, and accession data: PSMD4
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H M R Mk (Hm) (X) (Z) (B) (Dg) (Pg) (Hr) | Endogenous | 50 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Hm=Hamster
Mk=Monkey
X=Xenopus
Z=Zebrafish
B=Bovine
Dg=Dog
Pg=Pig
Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 12441:
- Immunoprecipitation, Western Blotting
Specificity / Sensitivity
S5a/PSMD4 (D20B2) Rabbit mAb recognizes endogenous levels of total S5a/PSMD4 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human S5a/PSMD4 protein.
Western Blotting
Western blot analysis of extracts from various cell lines using S5a/PSMD4 (D20B2) Rabbit mAb.
Western Blotting
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human S5a/PSMD4 (hS5a/PSMD4-Myc/DDK; +), using S5a/PSMD4 (D20B2) Rabbit mAb.
IP
Immunoprecipitation of S5a/PSMD4 from 293T cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or S5a/PSMD4 (D20B2) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using S5a/PSMD4 (D20B2) Rabbit mAb.
Background
S5a (PSMD4) is a subunit of the 19S regulatory proteasome complex functioning in ubiquitinated-protein targeting and degradation (1). S5a contains two polyubiquitin binding motifs (UIM) that bind multiubiquitin chains by hydrophobic interaction (2,3). In addition to ubiquitin, the UIM of S5a shows high affinity to a ubiquitin-like domain present in many proteins. S5a binds to these types of proteins directly and mediates their targeting to the proteasome for degradation (4,5).
- Deveraux, Q. et al. (1995) J. Biol. Chem. 270, 23726-23729.
- Young, P. et al. (1998) J. Biol. Chem. 273, 5461-5467.
- Beal, R. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 861-866.
- Fujiwara, K. et al. (2004) J. Biol. Chem. 279, 4760-4767.
- Sakata, E. et al. (2003) EMBO Rep. 4, 301-306.
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
