Product Pathways - Protein Stability
S5a/PSMD4 (D20B2) Rabbit mAb #12441
|12441S||100 µl (10 western blots)||---||In Stock||---|
|12441||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||50||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Species predicted to react based on 100% sequence homology: Hamster, Xenopus, Zebrafish, Bovine, Dog, Pig, Horse.
Specificity / Sensitivity
S5a/PSMD4 (D20B2) Rabbit mAb recognizes endogenous levels of total S5a/PSMD4 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human S5a/PSMD4 protein.
Western blot analysis of extracts from various cell lines using S5a/PSMD4 (D20B2) Rabbit mAb.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human S5a/PSMD4 (hS5a/PSMD4-Myc/DDK; +), using S5a/PSMD4 (D20B2) Rabbit mAb.
Immunoprecipitation of S5a/PSMD4 from 293T cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or S5a/PSMD4 (D20B2) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using S5a/PSMD4 (D20B2) Rabbit mAb.
S5a (PSMD4) is a subunit of the 19S regulatory proteasome complex functioning in ubiquitinated-protein targeting and degradation (1). S5a contains two polyubiquitin binding motifs (UIM) that bind multiubiquitin chains by hydrophobic interaction (2,3). In addition to ubiquitin, the UIM of S5a shows high affinity to a ubiquitin-like domain present in many proteins. S5a binds to these types of proteins directly and mediates their targeting to the proteasome for degradation (4,5).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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