Cell Signaling Technology

Product Pathways - Autophagy Signaling

MTMR3 Antibody #12443

Applications Reactivity Sensitivity MW (kDa) Source
W H (Mk) Endogenous 130-150 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

MTMR3 Antibody recognizes endogenous levels of total MTMR3 protein. This antibody also cross-reacts with a protein of unknown origin at 48 kDa.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro600 of human MTMR3 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MTMR3 Antibody.

Background

Myotubularin-related proteins are a family of phosphatases with emerging roles in cellular signaling and membrane trafficking (1,2). MTMR3 (Myotubularin-related protein 3), also known as FYVE-DSP1, contains an amino terminal pleckstrin homology (PH) domain and a carboxyl terminal FYVE domain. MTMR3 was first reported as a dual-specific phosphatase, having phosphatase activity toward phosphorylated serine, threonine, and tyrosine residues (3). Subsequent research studies reported that MTMR3 has phosphatase activity toward phosphoinositides, including phosphatadylinositol-3-phosphate (PI3P) and phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2) (4). Accumulation of PI3P by the class III phosphoinositide 3-kinase Vps34 is a key element in autophagosome formation (5). Inhibition of PI3P by MTMR3 can play an important role in suppressing autophagsome formation (6).

  1. Robinson, F.L. and Dixon, J.E. (2006) Trends Cell Biol 16, 403-12.
  2. Laporte, J. et al. (2002) Biochem Biophys Res Commun 291, 305-12.
  3. Zhao, R. et al. (2000) Biochem Biophys Res Commun 270, 222-9.
  4. Walker, D.M. et al. (2001) Curr Biol 11, 1600-5.
  5. Simonsen, A. and Tooze, S.A. (2009) J Cell Biol 186, 773-82.
  6. Taguchi-Atarashi, N. et al. (2010) Traffic 11, 468-78.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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