Cell Signaling Technology

Product Pathways - Neuroscience

CK1ε Antibody #12448

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Mk Endogenous 43 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CK1ε Antibody recognizes endogenous levels of total CK1ε protein. This antibody does not cross-react with CK1δ.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala349 of human CK1ε protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc-tagged full-length human CK1δ (CK1δ-Myc/DDK; +) or Myc-tagged full-length human CK1ε (CK1ε-Myc/DDK; +), using CK1ε Antibody (upper) or Myc-Tag (71D10) Rabbit mAb #2278 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CK1ε Antibody.

Background

Casein Kinase I (CK1 or CKI) is the name given to a family of kinases consisting of multiple isoforms (α, α', β, γ1-3, δ, and ε) with a conserved N-terminal kinase domain and a variable C-terminal sequence that determines subcellular localization and regulates enzyme activity (1-3). Indeed, multiple inhibitory autophosphorylation sites have been identified near the C terminus of CK1ε (3). This ubiquitously expressed family of protein kinases has been implicated in multiple processes including DNA repair, cell morphology, and Wnt signaling (4). Perhaps the best understood role of CK1 is to provide the priming phosphorylation of β-catenin at Ser45 to produce the consensus GSK-3 substrate motif (S/T-X-X-X-pS) (4).

CK1ε is involved in many cellular processes such as differentiation (5-7), cell growth and apoptosis (8), and control of the circadian rhythm (9,10).

  1. Gross, S.D. and Anderson, R.A. (1998) Cell. Signal. 10, 699-711.
  2. Vancura, A. et al. (1994) J. Biol. Chem. 269, 19271-19278.
  3. Gietzen, K.F. and Virshup, D.M. (1999) J. Biol. Chem. 274, 32063-32070.
  4. Polakis, P. (2002) Curr. Biol. 12, R499-R501.
  5. Okamura, A. et al. (2004) Blood 103, 2997-3004.
  6. Swiatek, W. et al. (2006) J Biol Chem 281, 12233-41.
  7. Bischof, J. et al. (2011) PLoS One 6, e20857.
  8. Brockschmidt, C. et al. (2008) Gut 57, 799-806.
  9. Keesler, G.A. et al. (2000) Neuroreport 11, 951-5.
  10. Meng, Q.J. et al. (2008) Neuron 58, 78-88.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products