Product Pathways - Apoptosis
Puma (D30C10) Rabbit mAb #12450
|12450S||100 µl (10 western blots)||---||In Stock||---|
|12450||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Species predicted to react based on 100% sequence homology: Monkey.
Specificity / Sensitivity
Puma (D30C10) Rabbit mAb recognizes endogenous levels of total Puma protein. This antibody also cross-reacts with a protein of unknown origin at 60 kDa.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Puma protein.
Western blot analysis of extracts from A549 cells, untreated (-) or treated with Doxorubicin #5927 (500 nM, overnight; +), using Puma (D30C10) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from various cell lines using Puma (D30C10) Rabbit mAb.
Immunoprecipitation of Puma from RL-7 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Puma (D30C10) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Puma (D30C10) Rabbit mAb.
Puma (p53 upregulated modulator of apoptosis) is a "BH3-only" Bcl-2 family member originally identified in differential gene expression studies as a p53-inducible gene (1,2). The "BH3-only" family members include Bad, Bid, Bik, Hrk, Bim, and Noxa, all of which contain a BH3 domain but lack other conserved domains, BH1 and BH2, and generally promote apoptosis by binding to and antagonizing anti-apoptotic Bcl-2 family members through BH3 domain interactions (3). Two BH3-containing proteins are produced from the puma gene, Puma-α and Puma-β, both of which are induced by p53, bind Bcl-2 and Bcl-xL, localize to the mitochondria, and promote cyctochrome c release and apoptosis (1,2). Puma plays a critical role in the p53 tumor suppressor pathway. Targeted disruption of the puma gene impairs p53-mediated apoptosis and tumor suprression (4-7). Puma knockout mice show defects from multiple apoptotic stimuli, including ionizing irradiation, deregulated c-Myc expression, and cytokine withdrawal (4).
- Yu, J. et al. (2001) Mol. Cell 7, 673-682.
- Nakano, K. and Vousden, K.H. (2001) Mol. Cell 7, 683-694.
- Bouillet, P. and Strasser, A. (2002) J. Cell Sci. 115, 1567-1574.
- Jeffers, J.R. et al. (2003) Cancer Cell 4, 321-328.
- Hemann, M.T. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 9333-9338.
- Yu, J. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 1931-1936.
- Villunger, A. et al. (2003) Science 302, 1036-1038.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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