Product Pathways - Translational Control
EDC3 Antibody #12469
|12469S||100 µl (10 western blots)||---||In Stock||---|
|12469||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||62||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
EDC3 Antibody recognizes endogenous levels of total EDC3 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly322 of human EDC3 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from Jurkat, KARPAS-299 and Hepa 1-6 cells using EDC3 Antibody.
Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human EDC3 (hEDC3-Myc/DDK; +), using EDC3 Antibody (upper), DYKDDDDK Tag (9A3) Mouse mAb #8146 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunoprecipitation of EDC3 from Jurkat cell extracts using Normal Rabbit IgG #2729 (lane 2) or EDC3 Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using EDC3 Antibody.
Enhancer of mRNA decapping 3 (EDC3) was originally identified from Saccharomyces cerevisiae as a protein essential to mRNA decapping prior to 5’-3’ mRNA degradation (1). In human cells, EDC3 is found within cytoplasmic processing (P) bodies as part of complexes that include DCP1, DCP2, EDC4/Ge-1, and DDX6/RCK (2). EDC3 and DCP2 interact with TTP, an activator of AU-rich-element (ARE)-mediated decay pathway, to promote decapping and degradation of ARE mRNA (2). In addition, research studies indicate that EDC3 may play a role in the premature termination of RNA polymerase II transcription (3).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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