Product Pathways - Development
β-Catenin (D10A8) XP® Rabbit mAb (Sepharose Bead Conjugate) #12475
|12475S||400 µl (40 immunoprecipitations)||---||In Stock||---|
|12475||carrier free and custom formulation / quantity||email request|
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|IP||1:20||Human, Mouse, Rat, Monkey||Endogenous||92||Rabbit IgG|
Species cross-reactivity is determined by western blot using the unconjugated antibody.
Applications Key: IP=Immunoprecipitation
Species predicted to react based on 100% sequence homology: Zebrafish, Bovine, Pig, Guinea Pig, Horse.
Specificity / Sensitivity
β-Catenin (D10A8) XP® Rabbit mAb (Sepharose Bead Conjugate) recognizes endogenous levels of total β-catenin protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro714 of human β-catenin protein
Immunoprecipitation of β-catenin from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose Bead Conjugate) #3423 (lane 3) and β-Catenin (D10A8) XP® Rabbit mAb (Sepharose Bead Conjugate) (lane 2). Lane 1 is 10% input. Western blot analysis was performed using β-Catenin (L87A12) Mouse mAb #2698.
This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. β-Catenin (D10A8) XP® Rabbit mAb (Sepharose Bead Conjugate) is useful for the immunoprecipitation of β-catenin. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated β-Catenin (D10A8) XP® Rabbit mAb #8480.
β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).
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- Lin, C. et al. (2002) Cell 108, 837-847.
- Yanagawa, S. et al. (2002) EMBO J. 21, 1733-1742.
- Yost, C. et al. (1996) Genes Dev. 10, 1443-1454.
- Morin, P.J. (1997) Science 275, 1787-1790.
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.