Product Pathways - Cytoskeletal Signaling
GM130 (D6B1) XP® Rabbit mAb #12480
|12480S||100 µl (10 western blots)||---||In Stock||---|
|12480P||40 µl (4 western blots)||---||In Stock||---|
|12480||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Monkey||Endogenous||130||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
GM130 (D6B1) XP® Rabbit mAb recognizes endogenous levels of total GM130 protein. This antibody may cross-react with a protein of unknown origin at 30 kDa.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr185 of human GM130 protein.
Western blot analysis of extracts from various cell lines using GM130 (D6B1) XP® Rabbit mAb.
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Brefeldin A #9972 (200 μM, 30 min; right), using GM130 (D6B1) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Immunoprecipitation of GM130 protein from ZR-75-1 cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or GM130 (D6B1) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using GM130 (D6B1) XP® Rabbit mAb.
The Golgi apparatus functions in the modification, organization, and transport of proteins and membranes targeted to other parts of the cell, such as the plasma membrane, lysosomes, and endosomes. This regulated transport is important for appropriate protein localization, secretion, and signal transduction. Members of the Golgin family of proteins, including GM130, Giantin, p115, and GRASP65, are defined by their presence in the Golgi matrix and by their long coiled coil domains. Golgin function, which is regulated in part by small GTPases of the Rab and Arl families, includes establishing and maintaining Golgi structure and transport (reviewed in 1). The Golgi cisternae are stacked and linked laterally to form a ribbon. GRASP65 and GM130 are required for membrane fusion events that mediate ribbon formation during Golgi assembly. These lateral fusion events allow for uniform distribution of Golgi enzymes (2). GM130 and Giantin interact with the transport factor p115 to facilitate endoplasmic reticulum (ER)-Golgi transport (3). GM130 is also involved in the transport of the Ether-a-go-go-Related (hERG) potassium ion channel. Inappropriate hERG localization may be an underlying cause in Long QT syndrome, a hereditary and potentially fatal cardiac arrhythmia (4). Further, GM130 was implicated in signal transduction regulating invasion, migration, and cell polarization via its interaction with and activation of serine/threonine kinases YSK1 and Mst4 (5).
- Barr, F.A. and Short, B. (2003) Curr. Opin. Cell Biol. 15, 405-413.
- Puthenveedu, M.A. et al. (2006) Nat. Cell Biol. 8, 238-248.
- Alvarez, C. et al. (2001) J. Biol. Chem. 276, 2693-2700.
- Roti, E.C. et al. (2002) J. Biol. Chem. 277, 47779-47785.
- Preisinger, C. et al. (2004) J. Cell Biol. 164, 1009-1020.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.