Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

SirT6 (D8D12) Rabbit mAb #12486

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC H M R Mk Endogenous 42, 36 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

SirT6 (D8D12) Rabbit mAb recognizes endogenous levels of total SirT6 protein. This antibody does not cross-react with other sirtuin proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to full-length mouse SirT6 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SirT6 (D8D12) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HCT 116 cells, expressing either non-targeting shRNA (shNT) or shSirT6, using SirT6 (D8D12) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from SirT6 wild-type (WT) and knockout (KO) mouse embryonic fibroblasts (MEF) using SirT6 (D8D12) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). SirT6 WT and KO MEF were kindly provided by Dr. David Lombard, University of Michigan.


IP

IP

Immunoprecipitation of SirT6 from HCT 116 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 3) or SirT6 (D8D12) Rabbit mAb (lane 2). Lane 1 is 10% input. Western blot analysis was performed using SirT6 (D8D12) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HCT 116 cells, expressing either non-targeting shRNA (shNT) (left) or shSirT6 (right), using SirT6 (D8D12) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

Background

The Silent Information Regulator (Sir2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as class III histone deacetylases. The first discovered and best characterized of this family is Saccharomyces cerevisiae Sir2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging (1). SirT6, a mammalian homolog of Sir2, is a nuclear, chromatin-associated protein that promotes the normal maintenance of genome integrity mediated by the base excision repair (BER) pathway (2-4). The BER pathway repairs single-stranded DNA lesions that arise spontaneously from endogenous alkylation, oxidation, and deamination events. SirT6 deficient mice show increased sensitivity to DNA-damaging agents, including the alkylating agents MMS and H2O2 (2). In addition, these mice show genome instability with increased frequency of fragmented chromosomes, detached centromeres, and gaps (2). SirT6 may regulate the BER pathway by deacetylating DNA Polβ or other core components of the pathway (2).

  1. Guarente, L. (1999) Nat Genet 23, 281-5.
  2. Mostoslavsky, R. et al. (2006) Cell 124, 315-29.
  3. Liszt, G. et al. (2005) J Biol Chem 280, 21313-20.
  4. Michishita, E. et al. (2005) Mol Biol Cell 16, 4623-35.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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