Product Pathways - Akt Signaling

PTEN Blocking Peptide #1250

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Description

This peptide is used to block PTEN (138G8) Rabbit mAb # 9559 reactivity.

Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks PTEN (138G8) Rabbit mAb #9559 signal in immunohistochemistry.

Applications

Use as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry protocols.

Directions for Use

For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 µl total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the PTEN (138G6) Rabbit mAb #9559 data sheet.

Background

PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers (1). PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3-kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4,5). PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382 and Thr383) that regulate PTEN stability and may affect its biological activity (6,7). PTEN regulates p53 protein level and activity (8) and is involved in G protein coupled signaling during chemotaxis (9,10).

Applications: Use as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry protocols.Quality Control: The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks PTEN (138G8) Rabbit mAb # 9559 signal in immunohistochemistry.Directions for Use: For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 ul total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the PTEN (138G6) Rabbit mAb #9559 data sheet.

1. Cantley, L.C. and Neel, B.G. (1999) Proc. Natl. Acad. Sci. USA 96, 4240-4245.
2. Myers, M.P. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 9052-9057.
3. Myers, M.P. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 13513-13518.
4. Wan, X. and Helman, L.J. (2003) Oncogene 22, 8205-8211.
5. Wu, X. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 15587-15591.
6. Vazquez, F. et al. (2000) Mol. Cell. Biol. 20, 5010-5018.
7. Torres, J. and Pulido, R. (2001) J. Biol. Chem. 276, 993-998.
8. Freeman, D.J. et al. (2003) Cancer Cell 3, 117-130.
9. Funamoto, S. et al. (2002) Cell 109, 611-623.
10. Iijima, M. and Devreotes, P. (2002) Cell 109, 599-610.

Application References

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Companion Products

• 9559 PTEN (138G6) Rabbit mAb