Product Pathways - Cytoskeletal Signaling
EVL Antibody #12536
|12536S||100 µl (10 western blots)||---||In Stock||---|
|12536||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
EVL Antibody recognizes endogenous levels of total EVL protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser362 of human EVL protein. Antibodies are purified by protein A and peptide affinity chromatography.
Ena/VASP-like (EVL) protein is a member of the Ena/VASP family and is involved in actin-associated cytoskeleton remodeling and cell polarity activities including axon guidance and lamellipodia formation in migrating cells (1,2,3). The EVL protein sequence contains an N-terminal EVH1 domain, a Pro-rich SH3 binding domain, and a C-terminal EVH2 domain. EVL domain interactions with G- and F-actin mediates actin nucleation and polymerization (4). Research studies have shown that EVL also regulates DNA repair by direct interaction with RAD51 (5). EVL may function in the DSB repair pathway through the EVH2 domain, which possesses DNA-binding and RAD51 binding activity, thereby coordinating homologous DNA recombination (6,7). Research studies have shown EVL expression is up-regulated in human breast cancer associated with clinical stages and may be implicated in invasion and/or metastasis of human breast cancer (8).
- Michael, M. et al. (2010) Curr Biol 20, 783-91.
- Goh, K.L. et al. (2002) Curr Biol 12, 565-9.
- Lebrand, C. et al. (2004) Neuron 42, 37-49.
- Laurent, V. et al. (1999) J Cell Biol 144, 1245-58.
- Takaku, M. et al. (2009) J Biol Chem 284, 14326-36.
- Takaku, M. et al. (2011) J Biochem 149, 721-9.
- Takaku, M. et al. (2009) FEBS J 276, 5841-8.
- Hu, L.D. et al. (2008) Oncol Rep 19, 1015-20.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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