Product Pathways - Translational Control
SAM68 Antibody #12538
|12538S||100 µl (10 western blots)||---||In Stock||---|
|12538||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||68||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Species predicted to react based on 100% sequence homology: Hamster, Bovine, Dog, Pig, Guinea Pig, Horse.
Specificity / Sensitivity
SAM68 Antibody recognizes endogenous levels of total SAM68 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human SAM68 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Src associated in mitosis 68 kDa (SAM68) is a member of the signal transduction and activation of RNA (STAR) family of RNA binding proteins (1,2). SAM68 is a multi-functional protein with roles in signal transduction, cell cycle regulation, transcription, and alternative splicing (1-3). SAM68 acts as a scaffold protein mediating responses to various stimuli that activate receptors, such as the T-cell receptor and the insulin receptor, linking activation of various signal transduction pathways with post-transcriptional gene regulation (3). SAM68 is a target for phosphorylation by Src-related kinases and Erk1/2 mitogen-activated protein kinases. Phosphorylation of SAM68 regulates both its affinity for RNA and alternative splicing of RNAs like CD44 receptor mRNA (1,4). Many of the activities of SAM68 and its targets for alternative splicing have been implicated in the development and progression of spinal muscular atrophy and cancers such as prostate and breast carcinomas, where SAM68 is frequently upregulated (1,5-8).
- Bielli, P. et al. (2011) Endocr Relat Cancer 18, R91-R102.
- Lukong, K.E. and Richard, S. (2003) Biochim Biophys Acta 1653, 73-86.
- Najib, S. et al. (2005) Cell Mol Life Sci 62, 36-43.
- Matter, N. et al. (2002) Nature 420, 691-5.
- Pedrotti, S. et al. (2010) EMBO J 29, 1235-47.
- Busà, R. et al. (2007) Oncogene 26, 4372-82.
- Rajan, P. et al. (2008) J Pathol 215, 67-77.
- Song, L. et al. (2010) J Pathol 222, 227-37.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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