Product Pathways - Cytoskeletal Signaling
EML4 (D5V6M) Rabbit mAb #12548
|12548S||100 µl (10 western blots)||---||In Stock||---|
|12548||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||120||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin)
Specificity / Sensitivity
EML4 (D5V6M) Rabbit mAb recognizes endogenous levels of total EML4 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn165 of human EML4 protein.
Western blot analysis of extracts from various cell lines using EML4 (D5V6M) Rabbit mAb.
Immunoprecipitation of EML4 from 293 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or EML4 (D5V6M) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using EML4 (D5V6M) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using EML4 (D5V6M) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Echinoderm microtubule-associated protein-like 4 (EML4) is a 120 kDa microtubule-associated WD-repeat protein of the echinoderm microtubule-associated protein family (1). The expression of EML4 is necessary for correct intracellular microtubule network formation (2). EML4 protein expression is upregulated during mitosis and downregulated during the remaining cell cycle (1). During mitosis, EML4 is heavily phosphorylated and is associated with the mitotic spindle (2). The amino-terminal segment of EML4 is essential for microtubule association and function.
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.