Product Pathways - Transcription Factors
CXXC1 Antibody #12585
|12585S||100 µl (10 western blots)||---||In Stock||---|
|12585||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
CXXC1 Antibody recognizes endogenous levels of total CXXC1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp117 of human CXXC1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
The CXXC finger protein 1 (CXXC1, CGBP, CFP1) is a key subunit of the human SET1 histone methyltransferase complex (1,2) that methylates histone H3 at Lys4 to create a mark of transcriptionally active promoters (3,4). CXXC1 is enriched at CpG islands where it selectively binds non-methylated CpG motifs to provide a link between global H3K4 methylation and CpG islands (5). Research studies have revealed a role for CXXC1 in the maintenance of cytosine methylation through direct interaction with DNMT1 (6-9). The epigenetic functions of CXXC1 are critical for normal embryonic development. Targeted deletion of the murine Cxxc1 gene results in early embryonic lethality while Cxxc1-null embryonic stem (ES) cells exhibit increased apoptosis and fail to undergo differentiation in vitro following withdrawal of leukemia inhibitory factor LIF (6).
- Xu, C. et al. (2011) Nat Commun 2, 227.
- Lee, J.H. and Skalnik, D.G. (2005) J Biol Chem 280, 41725-31.
- Miller, T. et al. (2001) Proc Natl Acad Sci U S A 98, 12902-7.
- Clouaire, T. et al. (2012) Genes Dev 26, 1714-28.
- Thomson, J.P. et al. (2010) Nature 464, 1082-6.
- Carlone, D.L. et al. (2005) Mol Cell Biol 25, 4881-91.
- Tate, C.M. et al. (2009) Mol Cell Biol 29, 3817-31.
- Young, S.R. et al. (2006) J Biol Chem 281, 37034-44.
- Butler, J.S. et al. (2008) DNA Cell Biol 27, 533-43.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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