Product Pathways - Apoptosis
Lamin B1 (D4Q4Z) Rabbit mAb #12586
|12586S||100 µl (10 western blots)||---||In Stock||---|
|12586||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||68, 25||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Species predicted to react based on 100% sequence homology: Monkey, Pig.
Specificity / Sensitivity
Lamin B1 (D4Q4Z) Rabbit mAb recognizes endogenous levels of total lamin B1 protein. This antibody recognizes the 25 kDa lamin B1 amino terminal cleavage product produced during apoptosis.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu118 of human lamin B1 protein.
Western blot analysis of extracts from various cell lines using Lamin B1 (D4Q4Z) Rabbit mAb.
Lamins are nuclear membrane structural components that are important in maintaining normal cell functions, such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamins have been subdivided into types A and B. Type-A lamins consist of lamin A and C, which arise from alternative splicing of the lamin A gene LMNA. Lamin A and C are cleaved by caspases into large (41-50 kDa) and small (28 kDa) fragments, which can be used as markers for apoptosis (4,5). Type-B lamins consist of lamin B1 and B2, encoded by separate genes (6-8). Lamin B1 is also cleaved by caspases during apoptosis (9). Research studies have shown that duplication of the lamin B1 gene LMNB1 is correlated with pathogenesis of the neurological disorder adult-onset leukodystrophy (10).
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