Product Pathways - Transcription Factors
MITF (D5G7V) Rabbit mAb #12590
|12590S||100 µl (10 western blots)||---||In Stock||---|
|12590||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Hamster, Monkey||Endogenous||50-75||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, ChIP=Chromatin IP
Specificity / Sensitivity
MITF (D5G7V) Rabbit mAb recognizes endogenous levels of total MITF protein. The antibody is predicted to recognize all known isoforms.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly335 of human MITF protein.
Western blot analysis of extracts from various cell lines using MITF (D5G7V) Rabbit mAb.
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 SK-MEL-28 cells and either 10 μl of MITF (D5G7V) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human Tyrosinase Promoter Primers #12970, human VMD2 intron 1 primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Microphthalmia-associated transcription factor (MITF) is a basic helix-loop-helix leucine zipper transcription factor that is most widely known for its roles in melanocyte, ophthalmic, and osteoclast development (1-3). In humans, MITF can function as a melanoma oncogene (4) and mutations in the corresponding MITF gene are associated with Waardenburg syndrome type 2, an auditory-pigmentary syndrome characterized by developmental defects in cells derived from neural crest (5). At least 12 isoforms of MITF have been identified, which exhibit differential patterns of expression of cell and tissue types (6).
- Hershey, C.L. and Fisher, D.E. (2004) Bone 34, 689-96.
- Nomura, S. et al. (2001) J Bone Miner Metab 19, 183-7.
- Widlund, H.R. and Fisher, D.E. (2003) Oncogene 22, 3035-41.
- Levy, C. et al. (2006) Trends Mol Med 12, 406-14.
- Read, A.P. and Newton, V.E. (1997) J Med Genet 34, 656-65.
- Tachibana, M. (2000) Pigment Cell Res 13, 230-40.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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