Product Pathways - MAPK Signaling
HSP27 Antibody Sampler Kit #12594
|12594S||1 Kit (4 x 40 µl)||---||In Stock||---|
Already purchased this product? Write a Review.
|Kit Includes||Quantity||Applications||Reactivity||Homology†||MW (kDa)||Isotype|
|Phospho-HSP27 (Ser82) (D1H2) XP® Rabbit mAb #9709||40 µl||W, IHC-P, IF-IC, F||H, M||R, Hm, B, Dg, Hr||27||Rabbit IgG|
|HSP27 (G31) Mouse mAb #2402||40 µl||W, IHC-P, IF-IC||H, Mk||27||Mouse IgG1|
|Phospho-HSP27 (Ser15) Antibody #2404||40 µl||W||H, Mk||27||Rabbit|
|Phospho-HSP27 (Ser78) Antibody #2405||40 µl||W, IHC-P, F||H, Mk||27||Rabbit|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
|Anti-mouse IgG, HRP-linked Antibody #7076||100 µl||Horse|
†Species predicted to react based on 100% sequence homology.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Reactivity Key: H=Human, M=Mouse, Mk=Monkey
Western blot analysis of extracts from HeLa and COS cells, untreated (-) or treated (+) with Anisomycin #2222 or UV, using Phospho-HSP27 (Ser15) Antibody #2404 (upper) or HSP27 (G31) Mouse mAb #2402 (lower).
Western blot analysis of extracts from HeLa and COS cells, untreated (-) or treated (+) with either Anisomycin #2222 or UV, using Phospho-HSP27 (Ser78) Antibody #2405 (upper) or HSP27 (G31) Mouse mAb #2402 (lower).
Western blot analysis of extracts from HeLa and HT-29 cells, untreated (-) or treated (+) with either UV (40 mJ/cm2 with 30 min recovery) or Anisomycin #2222 (25 μg/mL, 30 min), using Phospho-HSP27 (Ser82) (D1H2) XP® Rabbit mAb #9709.
The HSP27 Antibody Kit provides an economical means to evaluate the activation status of the HSP27 protein. The kit contains enough primary antibody to perform four western blot experiments per primary antibody.
Specificity / Sensitivity
Each antibody in the HSP27 Antibody Sampler Kit recognizes endogenous levels of its specific target. Activation state antibodies detect their intended targets only when phosphorylated at the indicated site.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser82 of human HSP27 and to full-length human HSP27 protein. Polyclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Ser15 or Ser78 of human HSP27 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the HSP27 expression increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78, and Ser82 by MAPKAPK-2 as a result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization (5,6).
- Arrigo, A.P. and Landry, J. (1994) Cold Spring Harbor Laboratory Press, NY, 335-373.
- Landry, J. et al. (1992) J. Biol. Chem. 267, 794-803.
- Rouse, J. et al. (1994) Cell 78, 1027-1037.
- Rogalla, T. et al. (1999) J. Biol. Chem. 274, 18947-18956.
- Lavoie, J. et al. (1993) J. Biol. Chem. 268, 24210-24214.
- Rousseau, S. et al. (1997) Oncogene 15, 2169-2177.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 11900 PhosphoPlus® HSP27 (Ser82) Antibody Duet
- 6356 SignalSilence® HSP27 siRNA I
- 6526 SignalSilence® HSP27 siRNA II
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.