Product Pathways - Tyrosine Kinase / Adaptors
Phospho-FLT3 Antibody Sampler Kit #12596
|12596S||1 Kit (5 x 40 µl)||---||In Stock||---|
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|Kit Includes||Quantity||Applications||Reactivity||MW (kDa)||Isotype|
|Phospho-FLT3 (Tyr589/591) (30D4) Rabbit mAb #3464||40 µl||W||H, M||160||Rabbit IgG|
|Phospho-FLT3 (Tyr591) (33G6) Rabbit mAb #3474||40 µl||W||H, M||160||Rabbit|
|Phospho-FLT3 (Tyr842) (10A8) Rabbit mAb #4577||40 µl||W, IP||H, M||160||Rabbit IgG|
|Phospho-FLT3 (Tyr969) (C24D9) Rabbit mAb #3463||40 µl||W||H, M||160||Rabbit IgG|
|FLT3 (8F2) Rabbit mAb #3462||40 µl||W, IP, IHC-P||H, M||130 nonglycosylated form;160 glycosylated mature form||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: H=Human, M=Mouse
Western blot analysis of extracts from Baf3/FLT3 transfected cells and SEM leukemia cells using FLT3 (8F2) Rabbit mAb #3462.
Western blot analysis of cell extracts from Baf3/FLT3 cells, untreated (-) or treated with FLT3 ligand (FL; +), using Phospho-FLT3 (Tyr969) (C24D9) Rabbit mAb #3463 (upper) or FLT3 (8F2) Rabbit mAb Antibody #3462 (lower).
Western blot analysis of extracts from cells expressing various activated tyrosine kinase proteins using Phospho-FLT3 (Tyr589/591) (30D4) Rabbit mAb #3464 (upper) or Phospho-Tyrosine mAb (P-Tyr-100) #9411 (lower). Phospho-FLT3 (Tyr589/591) (30D4) Rabbit mAb specifically recognizes the activated FLT3 but not non-phosphorylated FLT3 protein.
Western blot analysis of SEM cell lysates, untreated (-) or treated with FLT3 inhibitor (+), using Phospho-FLT3 (Tyr591) Rabbit mAb #3474 (upper) and FLT3 (8F2) Rabbit mAb #3462 (lower).
Western blot analysis of cell extracts from SEM cells expressing constitutively activated FLT3 using Phospho-FLT3 (Tyr842) (10A8) Rabbit mAb #4577 (upper and middle). The middle blot was treated with calf intestinal phosphatase (CIP) before antibody probing. Total FLT3 (8F2) Rabbit mAb #3462 shown at the bottom.
The Phospho-FLT3 Antibody Sampler Kit provides an economical means of evaluating the FLT3 tyrosine kinase and several phosphorylation sites that are involved in its activation. The kit includes enough antibody to perform four western blot experiments with each primary antibody.
Specificity / Sensitivity
The phospho-FLT3 antibodies recognize the phosphorylated form of FLT3 at the indicated sites. The Phospho-FLT3 (Tyr591) (33G6) Rabbit mAb and Phospho-FLT3 (Tyr969) (C24D9) Rabbit mAb may cross-react with other tyrosine-phosphorylated proteins. The Phospho-FLT3 (Tyr842) (10A8) Rabbit mAb may cross-react with other tyrosine phosphorylated family members. The control FLT3 antibody recognizes both the phosphorylated and nonphosphorylated forms of this kinase.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Tyr589/591, Tyr591, Tyr842 or Tyr969 of human FLT3. The FLT3 antibody is produced by immunizing animals with a synthetic peptide surrounding Ser740 of human FLT3.
FMS-related tyrosine kinase 3 (FLT3, also called Flk2), is a member of the type III receptor tyrosine kinase family, which includes c-Kit, PDGFR and M-CSF receptors. FLT3 is expressed on early hematopoietic progenitor cells and supports growth and differentiation within the hematopoietic system (1,2). FLT3 is activated after binding with its ligand FL, which results in a cascade of tyrosine autophosphorylation and tyrosine phosphorylation of downstream targets (3). The p85 subunit of PI3 kinase, SHP2, GRB2 and Shc are associated with FLT3 after FL stimulation (4-6). Tyr589/591 is located in the juxtamembrane region of FLT3 and may play an important role in regulation of FLT3 tyrosine kinase activity. Somatic mutations of FLT3 consisting of internal tandem duplications (ITDs) occur in 20% of patients with acute myeloid leukemia (7).
- Shurin, M.R. et al. (1998) Cytokine Growth Factor Rev 9, 37-48.
- Naoe, T. et al. (2001) Cancer Chemother Pharmacol 48 Suppl 1, S27-30.
- Namikawa, R. et al. (1996) Stem Cells 14, 388-95.
- Beslu, N. et al. (1996) J Biol Chem 271, 20075-81.
- Zhang, S. and Broxmeyer, H.E. (2000) Biochem Biophys Res Commun 277, 195-9.
- Zhang, S. et al. (1999) J Leukoc Biol 65, 372-80.
- Mizuki, M. et al. (2000) Blood 96, 3907-14.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
Select rabbit monoclonal antibodies are developed, validated, and produced at CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and in some instances 7,429,487) from Epitomics, Inc.