Product Pathways - Chromatin Regulation / Epigenetics
HEXIM1 (D5Y5K) Rabbit mAb #12604
|12604S||100 µl (10 western blots)||---||In Stock||---|
|12604||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Monkey||Endogenous||60||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
HEXIM1 (D5Y5K) Rabbit mAb recognizes endogenous levels of total HEXIM1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly63 of human HEXIM1 protein.
Western blot analysis of extracts from various cell lines using HEXIM1 (D5Y5K) Rabbit mAb
Immunoprecipitation of HEXIM1 from HeLa cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or HEXIM1 (D5Y5K) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using HEXIM1 (D5Y5K) Rabbit mAb.
Confocal immunofluorescent analysis of HeLa cells using HEXIM1 (D5Y5K) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).
Hexamethylene bis-acetamide-inducible protein 1 (HEXIM1) was originally identified in vascular smooth muscle cells as a protein that is upregulated upon treatment with the differentiating agent hexamethylene bisacetamide (1). HEXIM1 binds 7SK RNA, a highly abundant non-coding RNA, and together they act as a potent inhibitor of positive transcription elongation factor b (P-TEFb) (2,3). P-TEFb phosphorylates the C-terminal domain of the largest subunit of RNA polymerase II and is an important regulator of transcription elongation (4-8). 7SK RNA-bound HEXIM1 interacts with the cyclin T1 subunit of P-TEFb, sequestering P-TEFb in an inactive form leading to transcription inhibition (2,3). The regulation of the relative ratio of inactive to active P-TEFb in the cell by HEXIM1/7SK RNA is thought to play a critical role in regulation of a wide range of cellular gene expression programs such as estrogen and glucocorticoid receptor regulated genes (9-12).
- Ouchida, R. et al. (2003) Genes Cells 8, 95-107.
- Michels, A.A. et al. (2004) EMBO J 23, 2608-19.
- Yik, J.H. et al. (2003) Mol Cell 12, 971-82.
- Buratowski, S. (2009) Mol Cell 36, 541-6.
- Lenasi, T. and Barboric, M. RNA Biol 7, 145-50.
- Pirngruber, J. et al. (2009) Cell Cycle 8, 3636-42.
- Wada, T. et al. (1998) EMBO J 17, 7395-403.
- Yamada, T. et al. (2006) Mol Cell 21, 227-37.
- Peterlin, B.M. et al. (2012) Wiley Interdiscip Rev RNA 3, 92-103.
- Ketchart, W. et al. (2011) Oncogene 30, 3563-9.
- Ogba, N. et al. (2008) Cancer Res 68, 7015-24.
- Shimizu, N. et al. (2005) Proc Natl Acad Sci U S A 102, 8555-60.
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