Product Pathways - Lymphocyte Signaling
FoxP3 (D6O8R) XP® Rabbit mAb #12653
|12653S||100 µl (10 western blots)||---||In Stock||---|
|12653P||40 µl (4 western blots)||---||In Stock||---|
|12653||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse||Endogenous||45||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, F=Flow Cytometry
* Product-specific protocol.
Specificity / Sensitivity
FoxP3 (D6O8R) XP® Rabbit mAb recognizes endogenous levels of total FoxP3 protein. This antibody recognizes both human and mouse FoxP3 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro44 of mouse FoxP3 protein.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with mouse FoxP3 (mFoxP3; +), COS-7 cells, transfected with human FoxP3 (hFoxP3; +), and mouse thymus tissue using FoxP3 (D6O8R) XP® Rabbit mAb.
Western blot analysis of extracts from CD4-positive (+) and CD4-negative (-) mouse splenocytes using FoxP3 (D6O8R) XP® Rabbit mAb (upper) and β-actin (D6A8) Rabbit mAb #8457 (lower).
Flow cytometric analysis of murine spleen lymphocytes using a CD25 antibody and FoxP3 (D6O8R) XP® Rabbit mAb. Anti-rabbit IgG (H+L), (F(ab')2 ) Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. Analysis was performed on a gated CD4+ T cell population.
Forkhead box (Fox) proteins are a family of evolutionarily conserved transcription factors containing a sequence known as Forkhead box or winged helix DNA binding domain (1). The human genome contains 43 Fox proteins that are divided into subfamilies. The FoxP subfamily has four members, FoxP1 - FoxP4, which are broadly expressed and play important roles in organ development, immune response and cancer pathogenesis (2-4). The FoxP subfamily has several characteristics that are atypical among Fox proteins: their Forkhead domain is located at the carboxy-terminal region and they contain motifs that promote homo- and heterodimerization. FoxP proteins usually function as transcriptional repressors (4,5).
FoxP3 is crucial for the development of T cells with regulatory properties (Treg) (6). Mutations in FoxP3 are associated with immune dysregulation, polyendocrinopathy, enteropathy, and X-linked syndrome (IPEX) (7), while overexpression in mice causes severe immunodeficiency (8). Research studies have shown that FoxP3 functions as a tumor suppressor in several types of cancer (9-11).
- Myatt, S.S. and Lam, E.W. (2007) Nat Rev Cancer 7, 847-59.
- Shu, W. et al. (2001) J Biol Chem 276, 27488-97.
- Lu, M.M. et al. (2002) Gene Expr Patterns 2, 223-8.
- Koon, H.B. et al. (2007) Expert Opin Ther Targets 11, 955-65.
- Li, S. et al. (2004) Mol Cell Biol 24, 809-22.
- Ochs, H.D. et al. (2007) Immunol Res 38, 112-21.
- Bennett, C.L. et al. (2001) Nat Genet 27, 20-1.
- Kasprowicz, D.J. et al. (2003) J Immunol 171, 1216-23.
- Zuo, T. et al. (2007) Cell 129, 1275-86.
- Zuo, T. et al. (2007) J Clin Invest 117, 3765-73.
- Wang, L. et al. (2009) Cancer Cell 16, 336-46.
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.