Product Pathways - Translational Control
THOC4/ALY (D3R4R) Rabbit mAb #12655
|12655S||100 µl (10 western blots)||---||In Stock||---|
|12655||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||27||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
THOC4/ALY (D3R4R) Rabbit mAb recognizes endogenous levels of total THOC4/ALY protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala192 of human THOC4/ALY protein.
Western blot analysis of extracts from various cell lines using THOC4/ALY (D3R4R) Rabbit mAb.
Immunoprecipitation of THOC4/ALY from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or THOC4/ALY (D3R4R) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using THOC4/ALY (D3R4R) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as a secondary antibody.
Confocal immunofluorescent analysis of HeLa cells using THOC4/ALY (D3R4R) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).
mRNA export is a process that is tightly coupled to mRNA splicing (1-4). Splicing and packaging of mRNAs in the form of an mRNA-protein complex (mRNP) leads to the recruitment of the mRNA export adaptor THOC4/ALY, via its interaction with the splicing factor UAP56, forming a large complex termed the transcription-export complex (TREX) (1,2,5). THOC4/ALY then directly interacts with NXF1/TAP, a part of the heterodimer that targets the mRNP to the nuclear pore complex, resulting in the shuttling of mRNP out of the nucleus and into the cytoplasm (1-3,6).
- Katahira, J. (2012) Biochim Biophys Acta 1819, 507-13.
- Siddiqui, N. and Borden, K.L. (2012) Wiley Interdiscip Rev RNA 3, 13-25.
- Reed, R. and Magni, K. (2001) Nat Cell Biol 3, E201-4.
- Masuda, S. et al. (2005) Genes Dev 19, 1512-7.
- Luo, M.L. et al. (2001) Nature 413, 644-7.
- Kang, Y. and Cullen, B.R. (1999) Genes Dev 13, 1126-39.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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