Product Pathways - Growth Factors/Cytokines
MouseHis6Interleukin-21 (mHis6IL-21) #12670
|12670LF||50 µg (Carrier Free)||---||In Stock||---|
|12670LC||50 µg (With Carrier)||---||In Stock||---|
|12670SF||10 µg (Carrier Free)||---||In Stock||---|
|12670SC||10 µg (With Carrier)||---||In Stock||---|
Already purchased this product? Write a Review.
Recombinant mouse His6IL-21 (mHis6IL-21) Pro25-Ser146 (Accession #NP_068554.1) was expressed in human 293 cells at Cell Signaling Technology.
Recombinant His6-tagged IL-21 has a calculated MW of 16,952. DTT-reduced and non-reduced protein migrate as 18-28 kDa polypeptides. Heterogeneity in SDS PAGE is due to glycosylation. The expected amino terminus of recombinant mHis6IL-21 was verified by amino acid sequencing.
>95% as determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant mHis6IL-21. All lots are greater than 95% pure.
The bioactivity of recombinant mHis6IL-21 was determined in a B9 cell proliferation assay. The ED50 of each lot is between and 20-80 pg/ml.
The purity of recombinant mHis6 IL-21 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mHis6 IL-21 and staining overnight with Coomassie Blue.
Less than 0.01 ng endotoxin/1 μg mHis6IL-21.
With carrier: Lyophilized from a 0.22 μm filtered 10 mM HEPES pH 7.0 solution containing 20 μg BSA per 1 μg mHis6IL-21. Carrier free: Lyophilized from a 0.22 μm filtered 10 mM HEPES pH 7.0 solution.
IL-21 is a pleiotropic type I cytokine that is produced by CD4+ T cells, Th17 cells and NKT cells (1,2). The IL-21 receptor is a heterodimer that consists of an IL-21R and the common γ chain, γc (1). IL-21/IL-21R signaling involves activation of JAKs1/3, STAT3 and, to a lesser extent, STAT5 and STAT1 (1,2). IL-21 promotes B cell proliferation, survival and differentiation into plasma cells (3,4). IL-21 activates the cytolytic functions of CD8+ T cells and NK cells, and has been shown in mouse models to play a key role in immunity to chronic viral infections and promote anti-tumor immunity (5-7).
- Leonard, W.J. et al. (2008) J Leukoc Biol 84, 348-56.
- Spolski, R. and Leonard, W.J. (2008) Curr Opin Immunol 20, 295-301.
- Parrish-Novak, J. et al. (2000) Nature 408, 57-63.
- Parrish-Novak, J. et al. (2002) J Leukoc Biol 72, 856-63.
- Pan, X.C. et al. (2013) Oncol Lett 5, 90-96.
- Fröhlich, A. et al. (2009) Science 324, 1576-80.
- Yi, J.S. et al. (2009) Science 324, 1572-6.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.