Product Pathways - Tyrosine Kinase / Adaptors
Phospho-EphA2 (Tyr588) (D7X2L) Rabbit mAb #12677
|12677S||100 µl (10 western blots)||---||In Stock||---|
|12677||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey.
Specificity / Sensitivity
Phospho-EphA2 (Tyr588) (D7X2L) Rabbit mAb recognizes endogenous levels of EphA2 protein only when phosphorylated at Tyr588. This antibody may cross-react with other overexpressed phosphotyrosine proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr588 of human EphA2 protein.
Western blot analysis of extracts from SNB19 cells, untreated (-) or EphrinA1-Fc ligand-treated (100 ng/ml, 5 min; +), using Phospho-EphA2 (Tyr588) (D7X2L) Rabbit mAb (upper) or EphA2 (D4A2) XP® Rabbit mAb #6997 (lower).
Immunoprecipitation of phospho-EphA2 (Tyr588) from EphrinA1-Fc ligand-treated (100 ng/ml, 5 min) SNB19 cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Phospho-EphA2 (Tyr588) (D7X2L) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-EphA2 (Tyr588) (D7X2L) Rabbit mAb.
The Eph receptors are the largest known family of receptor tyrosine kinases (RTKs). They can be divided into two groups based on sequence similarity and on their preference for a subset of ligands: EphA receptors bind to a glycosylphosphatidylinositol-anchored ephrin A ligand; EphB receptors bind to ephrin B proteins that have a transmembrane and cytoplasmic domain (1,2). Research studies have shown that Eph receptors and ligands may be involved in many diseases including cancer (3). Both ephrin A and B ligands have dual functions. As RTK ligands, ephrins stimulate the kinase activity of Eph receptors and activate signaling pathways in receptor-expressing cells. The ephrin extracellular domain is sufficient for this function as long as it is clustered (4). The second function of ephrins has been described as "reverse signaling", whereby the cytoplasmic domain becomes tyrosine phosphorylated, allowing interactions with other proteins that may activate signaling pathways in the ligand-expressing cells (5). Various stimuli can induce tyrosine phosphorylation of ephrin B, including binding to EphB receptors, activation of Src kinase, and stimulation by PDGF and FGF (6). Tyr324 and Tyr327 have been identified as major phosphorylation sites of ephrin B1 in vivo (7).
Phosphorylation of Tyr594 was identified in several tumor cell lines (8,9). It was demonstrated that phosphorylated Tyr588 and Tyr594 of EphA2 provide binding sites for guanine nucleotide exchange factors Vav2 and Vav3, which may be involved in regulation of cell migration (10).
- Wilkinson, D.G. (2000) Int Rev Cytol 196, 177-244.
- Klein, R. (2001) Curr Opin Cell Biol 13, 196-203.
- Dodelet, V.C. and Pasquale, E.B. (2000) Oncogene 19, 5614-9.
- Holder, N. and Klein, R. (1999) Development 126, 2033-44.
- Brückner, K. et al. (1997) Science 275, 1640-1643.
- Palmer, A. et al. (2002) Mol. Cell 9, 725-737.
- Kalo, M.S. et al. (2001) J Biol Chem 276, 38940-8.
- Guo, A. et al. (2008) Proc Natl Acad Sci U S A 105, 692-7.
- Rikova, K. et al. (2007) Cell 131, 1190-203.
- Fang, W.B. et al. (2008) J Biol Chem 283, 16017-26.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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