Product Pathways - Lineage Markers
Endodermal Lineage Marker Antibody Sampler Kit #12693
|12693S||1 Kit (8 x 40 µl)||---||In Stock||---|
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|Kit Includes||Quantity||Applications||Reactivity||Homology†||MW (kDa)||Isotype|
|AFP (D12C1) Rabbit mAb #4448||40 µl||W, IP||H||Mk||65||Rabbit IgG|
|N-Cadherin Antibody #4061||40 µl||W||H, M, R, Mk||Dg||140||Rabbit|
|EOMES Antibody #4540||40 µl||W||M||H, R, Mk||70||Rabbit|
|FoxA2/HNF3β (D56D6) XP® Rabbit mAb #8186||40 µl||W, IP, IF-IC||H, M, R||50||Rabbit IgG|
|GATA-6 (D61E4) XP® Rabbit mAb #5851||40 µl||W, IF-IC||H||M, R, Dg, Pg||55||Rabbit IgG|
|HNF4α (C11F12) Rabbit mAb #3113||40 µl||W, IHC-P, IF-IC||H||52||Rabbit IgG|
|PDGF Receptor α (D13C6) XP® Rabbit mAb #5241||40 µl||W, IHC-P, IF-IC, F||H||190||Rabbit IgG|
|Sall4 (D16H12) Rabbit mAb #8459||40 µl||W, IF-IC||H||80, 142||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
†Species predicted to react based on 100% sequence homology.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey
Western blot analysis of extract from Hep G2 cells using HNF4α (C11F12) Rabbit mAb #3113.
Western blot analysis of extracts from various cell lines using N-cadherin Antibody #4061.
Western blot analysis of extracts from Hep G2 cells using AFP (D12C1) Rabbit mAb #4448.
Western blot analysis of extracts from E6.5 and E10.5 stage mouse embryos using EOMES Antibody #4540.
Western blot analysis of extracts from various cell lines using PDGF Receptor α (D13C6) XP® Rabbit mAb #5241 (upper), PDGF Receptor β (28E1) Rabbit mAb #3169 (middle), and β-Actin Antibody #4967 (lower).
Western blot analysis of extracts from Huh7 and 293 cells using GATA-6 (D61E4) XP® Rabbit mAb #5851.
Western blot analysis of extracts from Hep G2 cells using FoxA2/HNF3β (D56D6) XP® Rabbit mAb #8186.
The Endodermal Lineage Marker Antibody Sampler Kit provides an economical means of evaluating proteins expressed during endoderm development. This kit contains enough antibody to perform four western blot experiments per primary antibody.
Specificity / Sensitivity
Each antibody recognizes endogenous total levels of its specific target protein. Sall4 (D16H12) Rabbit mAb recognizes endogenous levels of total Sall4A and Sall4B proteins.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human AFP protein, residues surrounding Gly138 of human FoxA2/HNF3β protein, residues near the amino terminus of human GATA-6 protein, the sequence of human HNF4α protein, a recombinant protein corresponding to the PDGF receptor α extracellular domain, or residues surrounding Ala311 of human Sall4 protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human N-cadherin protein or near the amino terminus of human EOMES protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.
Two endodermal lineages develop during mammalian embryogenesis, the primitive endoderm of the blastocyst stage embryo and the definitive endoderm at gastrulation. The primitive endoderm gives rise to extra-embryonic lineages encompassing the visceral and the parietal endoderm. The definitive endoderm contributes to the respiratory and gastrointestinal tracts by forming the epithelial lining of the trachea, esophagus, lungs, stomach and intestines, and is a major component of many glands, including thyroid, thymus, pancreas and liver (1). Understanding molecular mechanisms that regulate early endodermal fates is seminal for the advance of stem cell research as they connect the transition from pluripotency to endoderm specification during mammalian development and contribute to the generation of clinically relevant cell types. FoxA2/HNF3β is a transcription factor essential for development of the endoderm and midline structures in mouse embryos (2,3). EOMES acts during gastrulation to promote the specification of the definitive endoderm (4). Markers of hepatic differentiation in the endoderm include expression of α-fetoprotein (AFP) and N-cadherin (5,6). HNF4α is involved in the differentiation of the visceral endoderm. GATA-6 lies upstream of HNF4 in a transcriptional cascade that regulates differentiation of the visceral endoderm and is also required for the establishment of the endodermally derived bronchial epithelium (7). Sall4 is required for the formation of the primitive endoderm from inner cell mass. It has been reported that extra-embryonic stem cell lines cannot be formed in Sall4-deficient blastocysts (8). PDGF receptor α is expressed in primitive endoderm derivatives throughout embryogenesis (9).
- Wells, J.M. and Melton, D.A. (1999) Annu Rev Cell Dev Biol 15, 393-410.
- Weinstein, D.C. et al. (1994) Cell 78, 575-88.
- Ang, S.L. and Rossant, J. (1994) Cell 78, 561-74.
- Costello, I. et al. (2011) Nat Cell Biol 13, 1084-91.
- Zhao, D. et al. (2009) PLoS One 4, e6468.
- Meier, V. et al. (2006) Comp Hepatol 5, 2.
- Morrisey, E.E. et al. (1998) Genes Dev 12, 3579-90.
- Elling, U. et al. (2006) Proc Natl Acad Sci U S A 103, 16319-24.
- Orr-Urtreger, A. et al. (1992) Development 115, 289-303.
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
Select rabbit monoclonal antibodies are developed, validated, and produced at CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and in some instances 7,429,487) from Epitomics, Inc.